| 引用本文:唐咏1，孙志佳1，侯乐2，温泽淮3，陈伯钧3，高彦利4，胡忠栋4.益气化瘀法改善慢性心力衰竭大鼠心肌能量代谢的研究〖JZ)〗〖HS)〗[J].世界中医药,2019,(05):. |
|
| 益气化瘀法改善慢性心力衰竭大鼠心肌能量代谢的研究〖JZ)〗〖HS)〗 |
| Study on Improving Energy Metabolism of Myocardium in Rats with Chronic Heart Failure with Qi-boosting and Stasis-dissolving Method |
| 投稿时间：2018-07-10 |
| DOI：10.3969/j.issn.1673-7202.2019.05.022 |
| 中文关键词: 益气化瘀法 黄芪注射液 丹参注射液 缬沙坦 慢性心力衰竭 心室肌细胞横截面积 心功能 心肌组织磷酸腺苷 |
| English Keywords:Qi-boosting and stasis-dissolving method Astragalus Injection Salvia Miltiorrhiza Injection Valsartan Chronic heart failure Cross section area of ventricular myocytes Cardiac function Adenosine phosphate in myocardial tissue |
| 基金项目:广东省中医药局一般项目（20171141） |
|
| 摘要点击次数: 635 |
| 全文下载次数: 470 |
| 中文摘要: |
| 目的：探讨益气化瘀法改善慢性心力衰竭大鼠心肌能量代谢的效果。方法：选取健康雄性Wistar大鼠60只，随机分为假手术组、缬沙坦组、中成药组、模型组，每组15只。建立气虚血瘀型慢性心力衰竭大鼠模型。术后第9周，气虚血瘀型慢性心力衰竭大鼠造模成功后，模型组、中成药组、缬沙坦组、假手术组，进行实验用药干预，模型组大鼠每天肌注生理盐水1 mL，连续4周；中成药组大鼠每天肌注黄芪注射液、丹参注射液各0.5 mL，连续4周；缬沙坦组按20 mg/（kg·d）的剂量灌胃，连续4周；假手术组每天肌注生理盐水1 mL。连续4周。比较各组大鼠心室肌细胞横截面积、心功能指标和心肌组织磷酸腺苷变化。结果：模型组、缬沙坦组和中成药组心室肌细胞横截面积大于假手术组，差异有统计学意义（P<0.05）；缬沙坦组和中成药组心室肌细胞横截面积小于模型组，差异有统计学意义（P<0.05）；中成药组心室肌细胞横截面积小于缬沙坦组，差异有统计学意义（P<0.05）。模型组、缬沙坦组和中成药组LVEF和LVFS低于假手术组，而LVESD和LVEDD高于假手术组，差异有统计学意义（P<0.05）；缬沙坦组和中成药组LVEF和LVFS高于模型组，而LVESD和LVED低于模型组，差异有统计学意义（P<0.05）；中成药组LVEF和LVFS高于缬沙坦组，差异有统计学意义（P<0.05）；而中成药组和缬沙坦组LVESD和LVED比较，差异无统计学意义（P>0.05）。模型组、缬沙坦组和中成药组ATP和ADP低于假手术组，差异有统计学意义（P<0.05）；缬沙坦组和中成药组ATP和ADP高于模型组，差异有统计学意义（P<0.05）；中成药组ATP和ADP高于缬沙坦组，差异有统计学意义（P<0.05）。结论：益气化瘀法可明显改善心力衰竭大鼠心肌能量代谢，具有重要研究意义。 |
| English Summary: |
| To discuss the effects of qi-boosting and stasis-dissolving method on myocardial energy metabolism in rats with chronic heart failure.Methods:A total of 60 healthy male Wistar rats were divided into four groups，the sham-operation group，the valsartan group，the Chinese patent medicine group and the model group，with 15 rats in each group.Rat model of chronic heart failure with qi deficiency and blood stasis was established.Nine weeks after the model establishment operation，the model group，the Chinese patent medicine group，the Valsartan group and the sham-operation group began receiving experimental medical intervention：rats in the model group received intramuscular injection with 1 mL of normal saline every day for 4 weeks consecutively; the rats in the Chinese patent medicine group were intramuscularly injected with 0.5 mL of Astragalus membranaceus injection and Salvia miltiorrhiza injection respectively every day for 4 weeks consecutively; the Valsartan group was administered 20 mg/(kg·d）of Valsartan every day for 4 weeks consecutively，and the sham-operation group received intramuscular saline 1 mL every day for 4 weeks consecutively.The cross-sectional area of ventricular myocytes，cardiac function and changes of myocardial adenosine monophosphate of rats in each group were compared.Results:The cross-sectional area of ventricular myocytes in the model group，the Valsartan group and the Chinese patent medicine group was higher than that of the sham-operation group(P<0.05); the cross-sectional area of ventricular myocytes in the Valsartan group and the Chinese patent medicine group was lower than that of the model group(P<0.05); the cross-sectional area of ventricular myocytes in the Chinese patent medicine group was lower than that of the Valsartan group(P<0.05).The levels of LVEF and LVFS in the model group，the Valsartan group and the Chinese medicine group were lower than that of the sham-operation group，while the levels of LVESD and LVEDD were higher than that of the control group(P<0.05); the levels of LVEF and LVFS in the Valsartan group and the Chinese patent medicine group were higher than that of the model group，and the levels of LVESD and LVED were lower than that of the model group(P<0.05); the levels of LVEF and the LVFS in the Chinese patent medicine group were higher than those of the Valsartan group(P<0.05).There was no statistical significance in the levels of LVESD and LVED between the Chinese patent medicine group and the Valsartan group(P>0.05).The levels of ATP and ADP were lower in the model group，the Valsartan group and the Chinese medicine group than in the sham-operation group(P<0.05)，and the levels of ATP and ADP in the Valsartan group and the Chinese patent medicine group were higher than that in the model group(P<0.05); and the levels of ATP and ADP in the Chinese patent medicine group were higher than those of the Valsartan group(P<0.05).Conclusion:The qi-boosting and stasis-dissolving method can significantly improve myocardial energy metabolism in rats with heart failure，and has important research significance. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
