| 引用本文:付杨1，杨莉莉1，韩凤娟2.理冲生髓饮对卵巢癌SKOV3细胞周期和凋亡影响[J].世界中医药,2019,(06):. |
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| 理冲生髓饮对卵巢癌SKOV3细胞周期和凋亡影响 |
| Effects of Lichong Shengsui Decoction on Cycle and Apoptosis of Ovarian Cancer SKOV3 Cells |
| 投稿时间：2018-11-01 |
| DOI：10.3969/j.issn.1673-7202.2019.06.008 |
| 中文关键词: 理冲生髓饮 卵巢癌SKOV3细胞 卵巢癌干细胞 细胞周期 细胞凋亡 |
| English Keywords:Lichong Shengsui Decoction Ovarian cancer SKOV3 cells Ovarian cancer stem cells Cell cycle Cell apoptosis |
| 基金项目:国家自然科学基金项目(81473717) |
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| 中文摘要: |
| 目的:探讨理冲生髓饮对卵巢癌SKOV3细胞周期和凋亡影响。方法:以卵巢癌SKOV3细胞为研究对象,采用CCK-8法检测不同浓度顺铂对SKOV3细胞抑制作用,Annexin V-FITC法检测SKOV3细胞凋亡情况,细胞周期与细胞凋亡检测试剂测定SKOV3细胞周期变化,流式细胞术检测SKOV3细胞中CD44+CD117+细胞比例。结果:与空白组比较,24 h和48 h顺铂组SKOV3细胞凋亡率明显增加,G0/G1期细胞比例降低,S期细胞比例升高,CD44+CD117+细胞比例明显增加,与顺铂组比较,24 h和48 h理冲生髓饮联合顺铂组SKOV3细胞凋亡率明显增加,G1期细胞比例降低,S期细胞比例升高,CD44+CD117+细胞比例明显降低,其中理冲生髓饮中剂量组联合顺铂组作用效果最强。结论:理冲生髓饮具有抗肿瘤作用,其机制可能是增加顺铂对卵巢癌SKOV3细胞凋亡,调控细胞周期阻滞,降低卵巢癌SKOV3细胞中干细胞数量。 |
| English Summary: |
| To explore the effects of Lichong Shengsui decoction on cycle and apoptosis of ovarian cancer SKOV3 cells.Methods:Taking ovarian cancer SKOV3 cells as the research objects，the inhibitory effect of different concentrations of cisplatin on SKOV3 cells was detected by CCK-8 method.The apoptosis of SKOV3 cells was detected by Annexin V-FITC method.The cycle changes of SKOV3 cells was detected with the cell cycle and apoptosis detection reagents，and the ratio of CD44+CD117+ cells in SKOV3 cells was detected by flow cytometry.Results:Compared with the blank group，the apoptosis rate of SKOV3 cells in the cisplatin group was significantly increased in the 24 h and 48 h.The proportion of cells in G0/G1 phase was decreased，the proportion of cells in S phase cells was increased，and the proportion of CD44+ CD117+ cells was significantly increased.Compared with the cisplatin group，the apoptosis rate of SKOV3 cells in Lichong Shengsui Decoction combined with cisplatin was significantly increased in the 24 h and 48 h.The proportion of cells in G1 phase was decreased，the proportion of cells in S phase was increased，and the proportion of CD44+CD117+cells was significantly decreased.Among them，the middle dose of Lichong Shengsui Decoction combined with cisplatin group had the strongest effects.Conclusion:Lichong Shengsui Decoction has anti-tumor effect，and its mechanism maybe increase the apoptosis of ovarian cancer SKOV3 cells by cisplatin，regulate cell cycle arrest and decrease the number of stem cells in ovarian cancer SKOV3 cells. |
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