To observe the effects of new anticancer prescription of Chanshan Hujin on the proliferation of lung adenocarcinoma A549 cells，and to investigate that whether it could induce the apoptosis of A519.Methods:A total of 30 SD rats were randomly divided into three groups of a serum containing drug group，a Xiao-Ai-Ping group and a normal saline group，with 10 rats in each group.Each group was intragastric administrated successively with the Decoction of Chan-Shan-Hu-Jin Prescription，Xiao-Ai-Ping water solvent and normal saline，to prepare the serum containing drug.A549 cell proliferation inhibition test(MTT test)，apoptosis assay and detection the expression of “death protease” Caspase-3 was carried out with serum containing drug.Results:Through the cell proliferation inhibition test(MTT test)，it was found that the group of Chan-Shan-Hu-Jin Prescription could inhibit the proliferation of A549 cells，and had positive correlation with dose.Compared with the negative group，the difference was significant(P<005).The difference was significant when compared with the Xiao-Ai-Ping group(P<005).The apoptotic detection was carried out by flow cytometry.It was found that the early apoptosis rate after the medium concentration treatment group and high concentration treatment group，and it had significant difference when compared with the negative group(P<005，P<005).Western-Blot test showed that the activation of “death protease” caspase-3 was up-regulated.Conclusion:The serum containing drug of Chan-Shan-Hu-Jin Prescription can inhibit the proliferation and induce the apoptosis of A549 cell，and has positive correlation with dose.