| 引用本文:张鹏，杨冬妹，聂波，桑勉.加减茵陈蒿汤联合布地奈德对哮喘大鼠气道反应性及肺组织中炎性细胞因子表达的影响[J].世界中医药,2019,(06):. |
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| 加减茵陈蒿汤联合布地奈德对哮喘大鼠气道反应性及肺组织中炎性细胞因子表达的影响 |
| Effects of Modified Yinchenhao Decoction Combined with Budesonide on Airway Responsiveness and Expression of Inflammatory Cytokines in Lung Tissue of Asthmatic Mice |
| 投稿时间：2019-01-23 |
| DOI：10.3969/j.issn.1673-7202.2019.06.010 |
| 中文关键词: 支气管哮喘 茵陈蒿汤 气道反应性 炎性反应 哮喘 大鼠 实验 |
| English Keywords:Bronchial asthma Yinchenhao decoction Airway reactivity Inflammation reaction Asthma Rats Experiment |
| 基金项目:国家自然科学基金青年基金项目(81704120)——基于CTCR1基因探讨加味芎蝎散对咳嗽变异性哮喘气道平滑肌干预作用及研究机制 |
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| 中文摘要: |
| 目的:研究加减茵陈蒿汤联合布地奈德对哮喘大鼠气道反应性及肺组织中炎性反应细胞因子表达的影响。方法:选择雄性SD大鼠作为实验动物,分为对照组、模型组、观察组,制作哮喘模型后给予加减茵陈蒿汤联合布地奈德治疗。测定气道阻力、肺泡灌洗液(BALF)中炎性细胞的数目以及肺组织中炎性细胞因子的表达水平。结果:模型组大鼠注射乙酰甲胆碱(MCh)后的气道阻力及BALF中中性粒细胞、嗜酸性粒细胞、淋巴细胞的数目及肺组织中白细胞介素(IL)-2、IL-6、干扰素-γ(IFN-γ)、嗜酸性粒细胞趋化因子(eotaxin)、CCL19、CCL21的mRNA表达水平均明显高于对照组(P<005);观察组大鼠注射MCh后的气道阻力及BALF中中性粒细胞、嗜酸性粒细胞、淋巴细胞的数目及肺组织中IL-2、IL-6、IFN-γ、eotaxin、CCL19、CCL21的mRNA表达水平均明显低于模型组(P<005)。结论:加减茵陈蒿汤联合布地奈德治疗哮喘大鼠能够降低气道反应性、抑制肺组织中的炎性反应,在哮喘治疗中展现出积极价值。 |
| English Summary: |
| To study the effects of modified Yinchenhao Decoction combined with budesonide on airway responsiveness and expression of inflammatory cytokines in lung tissue of asthmatic mice.Methods:Male SD rats were divided into a control group，a model group and a treatment group.After making asthma model，the mice were treated with modified Yinchenhao Decoction combined with budesonide.The airway resistance，the number of inflammatory cells in bronchoalveolar lavage fluid(BALF)and the expression of inflammatory cytokines in lung tissue were measured.Results:The airway resistance，the number of neutrophils，eosinophils and lymphocytes in BALF and the expression of interleukin-2(IL-2)，IL-6，interferon-γ(IFN-γ)，eotaxin，CCL19 and CCL21 in lung tissue of model group mice were significantly higher than those of control group(P<005).The airway resistance after MCh injection and the number of neutrophils，eosinophils and lymphocytes in BALF and the expression of IL-2，IL-6，IFN-γ，eotaxin，CCL19 and CCL21 in lung tissue of treatment group were significantly lower than those of the model group(P<005).Conclusion:Modified Yinchenhao Decoction combined with budesonide can reduce airway reactivity and inhibit inflammation in lung tissue of asthmatic mice，which shows positive value in the treatment of asthma. |
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