| 引用本文:周立，李淑荣，张娥.血府逐瘀汤对子宫肌瘤模型大鼠NOS、TNF-a、IL-2水平及子宫平滑肌厚度的影响[J].世界中医药,2019,(06):. |
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| 血府逐瘀汤对子宫肌瘤模型大鼠NOS、TNF-a、IL-2水平及子宫平滑肌厚度的影响 |
| Effect of Xuefu Zhuyu Decoction on NOS，TNF-a and IL-2 Levels and Uterine Smooth Muscle Thickness in Rats with Uterine Leiomyoma |
| 投稿时间：2018-10-31 |
| DOI：10.3969/j.issn.1673-7202.2019.06.013 |
| 中文关键词: 子宫肌瘤 大鼠 血府逐瘀汤 子宫平滑肌 一氧化氮酶 肿瘤坏死因子 白细胞介素-2 |
| English Keywords:Uterine fibroids Rat Xuefu Zhuyu Decoction NOS TNF-a IL-2 |
| 基金项目:河南省中医药科学研究专项课题(2018ZY2070)——温经通胞饮对输卵管阻塞性不孕患者血清IL-6和TNF-α的影响及临床疗效的研究 |
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| 中文摘要: |
| 目的:观察血府逐瘀汤对子宫肌瘤的疗效,并研究其对子宫平滑肌厚度的以及NOS、TNF-a、IL-2水平的影响。方法:将45只SD大鼠随机分为3组,分别是空白组、模型组、中药干预组,每组15只。其中模型组及中药干预组大鼠接收腹腔注射苯甲酸雌二醇建立子宫肌瘤大鼠模型,造模后中药干预组大鼠接收血府逐瘀汤灌胃,模型组及空白组大鼠接收等剂量生理盐水灌胃,连续灌胃15 d,治疗结束后测量各组大鼠子宫系数变化,采用HE染色法比较子宫平滑肌厚度变化,使用Western Blot测定大鼠子宫组织TNF-a浓度变化,Elisa法检测大鼠外周血清NOS、IL-2水平变化。结果:1)与空白对照组比较,模型组及中药干预组子宫系数增加,中药干预组明显较模型组小,差异有统计学意义(P<005);2)与空白对照组比较,模型组及中药干预组子宫平滑肌厚度增加,中药干预组明显较模型组减少,差异有统计学意义(P<005);3)空白组大鼠子宫平滑肌细胞排列整齐,平滑肌层薄;模型组大鼠平滑肌出现明显增生,细胞排列错乱,肌纤维疏松粗短,且在肌纤维束间出现明显结缔组织,纵行肌层和环行肌层均明显增厚。中药干预组较模型组有明显改善,平滑层细胞排列较整齐,出现轻度的细胞脱落现象,黏膜上皮细胞少量增生;4)模型组及中药干预组大鼠子宫组织TNF-a及外周血NOS浓度较空白对照组上调,IL-2浓度有所下降,其中中药干预组明显优于模型组,差异有统计学意义(P<005)。结论:血府逐瘀汤对子宫肌瘤有明显抑制效应,其作用机制可能与介导NOS、TNF-a、IL-2水平变化有关。 |
| English Summary: |
| To observe the curative effects of Xuefu Zhuyu Decoction on uterine leiomyoma，and study its effects on the thickness of uterine smooth muscle and the levels of NOS，TNF-a and IL-2.Methods:A total of 45 SD rats were randomly divided into three groups：a blank group，a model group and a traditional Chinese medicine intervention group，with 15 rats in each group.Rats in the model group and the intervention group received intraperitoneal injection of estradiol benzoate to establish a rat model of uterine fibroids.Rats in the intervention group received Xuefu Zhuyu Decoction for gastric lavage.Rats in the model group and the blank group received the same dose of normal saline for gastric lavage for 15 days.HE staining was used to compare the thickness of uterine smooth muscle，Western Blot was used to measure the concentration of TNF-a in rat uterus，and ELISA was used to detect the levels of NOS and IL-2 in rat peripheral serum.Results:1)Compared with the blank control group，the uterine coefficient of the model group and the Chinese medicine intervention group increased，the Chinese medicine intervention group was significantly smaller than the model group，and the difference was statistically significant(P<005).2)Compared with the blank control group，the thickness of uterine smooth muscle of the model group and the Chinese medicine intervention group increased，and the Chinese medicine intervention group was significantly less than the model group.The difference was statistically significant(P<005).3)The smooth muscle cells of the uterus in the blank group were well arranged and thin; the smooth muscle cells in the model group were obviously hyperplasia，disordered cell arrangement，loose and short myofibers，and there were obvious connective tissue between myofibers bundles，and the longitudinal and circular myofibrils were obviously thickened.Compared with the model group，the TCM intervention group showed obvious improvement，smooth layer cells arranged more orderly，slight cell exfoliation and a small amount of proliferation of mucosal epithelial cells.4)The concentration of TNF-a and NOS in uterine tissue and peripheral blood of the model group and TCM intervention group were increased，while the concentration of IL-2 was decreased.The TCM intervention group was significantly better than the model group，and the difference was statistically significant(P<005).Conclusion:Xuefu Zhuyu Decoction has obvious inhibitory effects on uterine leiomyoma，and its mechanism may be related to the changes of NOS，TNF-a and IL-2 levels. |
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