To observe the effects of Wenyang Bushen method on the levels of serum 25 hydroxyvitamin D3 in patients with plateau rheumatoid arthritis(RA).Methods:A total of 132 patients with plateau rheumatoid arthritis in Qinghai Hospital of Traditional Chinese Medicine from July 2015 to August 2017 were randomly divided into 2 groups66 cases in the control group were treated with conventional western medicine，and 66 cases in the study group were treated with Wenyang Bushen method on the basis of the control group.The 2 groups were treated continuously for 3 months.The clinical efficacy of the 2 groups after treatment was compared.Main clinical symptoms and signs，the health assessment questionnaire(HAQ)score and the DAS28 score in the 2 groups before and after the treatment were measured.The levels of laboratory indexes，serum 25 hydroxyvitamin D3 and hemorheological indexes in the 2 groups before and after treatment were detected.Results:After treatment，the total effective rate of clinical treatment in the observation group was significantly higher than that in the control group(P<005).Joint swelling index，joint pain index，joint tenderness index，morning stiffness time，joint functional classification and 20 m walking time in the 2 groups were significantly lower than those before treatment(P<005)，and the observation group were significantly lower than those in the control group(P<005).The HAQ score，DAS28 score，rheumatoid factor(RF)，C reactive protein(CRP)and erythrocyte sedimentation rate(ESR)levels in the 2 groups were significantly lower than those before treatment(P<005)，and the observation group were significantly lower than those in the control group(P<005).The level of serum 25 hydroxyvitamin D3 in the 2 groups was significantly higher than that before the treatment(P<005)，and the observation group was significantly higher than the control group(P<005).The levels of erythrocyte electrophoresis index，hematocrit，plasma viscosity，whole blood low shear viscosity，and whole blood high shear viscosity in the 2 groups were significantly lower than those before treatment(P<005)，and the observation group were significantly lower than those in the control group(P<005).Conclusion:The therapeutic effect of Wenyang Bushen method on plateau rheumatoid arthritis is satisfactory，and it can significantly increase the level of serum 25 hydroxyvitamin D3.