To observe the effect and the influence of Jianpi Zhidong Decoction combined with auricular point sticking on hypothalamic-pituitary-adrenal axis (HPA-axis) and neurotransmitter content of children with Tourette syndrome (TS). Methods:A total of 80 cases with TS who met the TS diagnostic criteria of the fourth edition of the American Psychiatric Diagnostic Manual were enrolled in this study, which were divided into observation group and control group with 40 cases were in each group according to random number table. Another 40 healthy cases were enrolled in the study as healthy control group. Observation group and control group were treated with haloperidol tablets. Observation group was treated with auricular point sticking, and control group was treated with Jianpi Zhidong Decoction combined with auricular point sticking. Both groups had been treated for 12 weeks. The central nervous system (CNS) neurotransmitter such as dopamine (DA), 5-hydroxytryptamine (5-HT) norepinephrine (NE), aspartate (ASP),γ-aminobutyric acid (GABA) and HPA-related hormone such as cortisol (COR) and adrenocorticotropic hormone (ATCH) were detected before and after treatment, which were compared with the healthy control group. Yale comprehensive twitch severity scale (TGTSS) score were calculated. All the cases had been followed at least 6 months, and then the clinical efficacy was assessed. Results:Before treatment, the DA, NE and GABA levels in the observation group and the control group were significantly lower than those in healthy control group. The difference was statistically significant (P<005). 5-HT and ASP levels in 2 groups were significantly higher than those in healthy control group. The difference was statistically significant (P<005). After treatment, the DA, 5-HT, ASP levels in observation group and control group were significantly lower than those before treatment. The difference was statistically significant (P<005). NE and GABA levels were significantly higher than those before treatment. The difference was statistically significant (P<005). DA, 5-HT, and ASP levels in the observation group were significantly lower than those in the control group. The difference was statistically significant (P<005). NE, GABA levels of observation group were significantly higher than that of control group. The difference was statistically significant (P<005). Before treatment, COR and ACTH levels in observation group and control group were significantly higher than those in healthy control group. The difference was statistically significant (P<005). After treatment, COR and ACTH levels in the observation group and the control group were significantly lower than those in healthy control group. The difference was statistically significant (P<005). COR and ACTH levels in the observation group was significantly lower than the control group. The difference was statistically significant (P<005). The movement and vocalization tics and tics total scores of the observation group and the control group were significantly decreased after treatment. The difference was statistically significant (P<005). The scores in the observation group were significantly lower than those in control group after treatment. The difference was statistically significant (P<005). The total clinical effective rates in control group and observation group were 85% and 975% respectively. The difference was statistically significant (P<005). Conclusion:The attack of TS is related to central nervous system imbalance and hyperactivity of HPA axis. Jianpi Zhidong Decoction combined with auricular point sticking can effectively reduce the level of HPA axis, regulate the imbalance of CNS neurotransmitter and improve the clinical therapeutic effects.