To observe the therapeutic effects of meridian acupuncture on patients with migraine，and to investigate the mechanism of the expression of calcitonin gene related peptide(CGRP)，plasma endothelin(ET)and five serotonin(5-HT).Methods:From February 2017 to February 2018，85 cases of migraine patients were diagnosed in the outpatient department of Neurology Department of Tianjin University of Chinese Medicine Affiliated Wuqing Traditional Chinese Hospital were selected and randomly divided into 43 cases in the observation group and 40 cases in the control group according to the random digital table method.After excluding the 5 cases of adverse reactions，the 2 groups had 40 cases in each.The observation group was given acupuncture at Jiaosun(SJ20)，Fengchi(GB20)，Waiguan(SJ5)，Yanglingquan(GB34)and Qiuxu(GB40)along shaoyang meridian，and the control group was given non-acupoint or non-meridian acupuncture therapy.The migraine scores and VAS(visual analog scale)were evaluated before and 05 h，1 h，2 h and 4 h after treatment.Radioimmunoassay was used to detect calcitonin gene related peptide(CGRP)and plasma endothelin(ET)and the five hydroxyserine(5-HT)was detected by using ELISA(enzyme-linked immunosorbent assay).The expression was observed before and 4 h after treatment.The mechanism of acupuncture along meridians was discussed.Finally，the adverse reactions during the study were recorded.Results:1)The clinical effective rate of acupuncture on migraine was 90% in the observation group and 6750% in the control group.The clinical efficiency of the observation group was better than that of the control group.The difference was statistically significant(P<005)after the chi square test.2)The scores of migraine and VAS of 05 h，1 h，2 h and 4 h were decreased than those before treatment(P<005).The migraine score and VAS score of 2 h and 4 h were lower than those in the control group after treatment(P<005).3)There was no significant difference in the expression of CGRP and ET before treatment in the two groups(P>005).Compared with before treatment，the expression level of CGRP and ET after the treatment of 4 h decreased in the 2 groups(P<005)，and the lower levels of CGRP and ET after treatment in the observation group were better than those of the control group(P<005).4)There was no significant difference in the expression of 5-HT before treatment in the 2 groups(P>005).Compared with before treatment，the expression level of 5-HT was up-regulated in the 2 groups after the treatment of 4 h(P<005)，and the observation group was superior to the control group(P<005).5)In the course of the study，5 patients had adverse reactions，of which there were 3 cases in the observation group(1 case of needle sickness，and 2 cases of local bleeding)，and 2 cases in the control group(1 case of needle sickness and 1 case of local bleeding).The patient with needle sickness can recover when withdrawing the needle lying on the back.Local bleeding was processed with local press for stopping bleeding.No serious adverse reactions occurred during the whole study.Conclusion:The immediate effect of meridian acupuncture on migraine is relieving the symptoms after 05 h's treatment，and the effect is best after treatment of 4 h.The immediate effect may be related to the up-regulation of CGRP and 5-HT expression and the down-regulation of ET expression.