世界中医药

目的：观察右归丸对功能性便秘大鼠胃肠动力的影响，同时探讨其肠神经递质5-羟色胺(5-HT)、一氧化氮(NO)、肠内P物质(SP)、血管活性肠肽(VIP)以及结肠组织水通道蛋白3（AQP3）浓度的变化。方法：选取60只功能性便秘模型大鼠并将其随机分为空白组、模型组以及干预组，每组20只。其中模型组及干预组大鼠接受复方地芬诺酯混悬液灌胃制备功能性便秘模型，随后对干预组大鼠进行右归丸悬浊液灌胃，共给药20 d。用活性炭进行灌胃计算各组胃排空率、小肠推进率；酶联免疫吸附试验法（ELISA）检测各组大鼠外周血肠神经递质SP、NO、5-HT、VIP水平变化；免疫蛋白质印迹（Western blotting）法观察各组结肠组织AQP3表达变化。结果：模型组及干预组大鼠造模开始后逐渐出现精神萎靡、喜卧、食欲减退、粪便量少、便质干硬等症状，甚至部分大鼠出现小便减少。经过干预后大鼠上述症状逐渐改善，甚至出现部分大鼠症状消失。模型组及干预组大鼠的胃排空率及小肠推进率明显减弱，与空白组比较，差异有统计学意义（P<0.05），其中干预组大鼠的胃排空率及小肠推进率明显较模型组快，差异有统计学意义（P<0.05）。模型组及干预组大鼠血清SP、VIP水平降低，NO及5-HT升高，与空白组比较，差异有统计学意义（P<0.05），其中干预组优于模型组，差异有统计学意义（P<0.05）。4）模型组及干预组大鼠结肠组织AQP3表达明显升高，与空白组比较，差异有统计学意义（P<0.05），其中干预组大鼠较模型组水平降低，差异有统计学意义（P<0.05）。结论：功能性便秘的发生可能与肠神经递质及水通道蛋白的异常有关，右归丸治疗功能性便秘可能通过调节SP、NO、5-HT、VIP及AQP3水平实现。

English Summary:

To observe the effects of Yougui Pills on gastrointestinal motility in rats with functional constipation,and to explore the concentration changes of intestinal neurotransmitters SP,NO,5-HT and VIP,as well as aquaporin 3(AQP3）in colon tissue.Methods：A total of 60 rats with functional constipation were randomly divided into blank group,model group and intervention group,with 20 rats in each group.The rats in the model group and the intervention group were given compound diphenoxylate suspension by gavage to prepare functional constipation models,and then the rats in the intervention group were given Yougui Pills suspension by gavage for 20 days.Gastric emptying rate and small intestinal propulsion rate in each group were calculated by gavage with activated carbon,levels of intestinal neurotransmitters SP,NO,5-HT and VIP in peripheral blood of rats were detected by enzyme-linked immunosorbent assay(ELISA),and expression of AQP3 in colon tissue of rats in each group was observed by Western blotting.Results：1）After the modeling,rats in the model group and the intervention group began to develop symptoms of listlessness,liking to lie down,loss of appetite,less fecal volume,dry stool,and even less urine in some rats.After the intervention,the above symptoms of rats were gradually improved,and symptoms of some rats disappeared. 2）The gastric emptying rate and small intestinal propulsion rate of the model group and the intervention group were significantly lower than those of the blank group,with statistically significant difference(P<0.05).The gastric emptying rate and small intestinal propulsion rate of the intervention group were significantly faster than those of the model group,with statistically significant difference(P<0.05). 3）In the model group and the intervention group,the levels of serum SP and VIP were decreased and the levels of NO and 5-HT were increased,compared with those in the blank group,with statistically significant difference(P<0.05),and those in the intervention group were superior to those in the model group,with statistically significant difference(P<0.05). 4）The expression of AQP3 in colon tissue of the model group and the intervention group was significantly higher than that of the blank group,with statistically significant difference(P<0.05),and the level of AQP3 in the intervention group was lower than that of the model group,with statistically significant difference(P<0.05).Conclusion：The occurrence of functional constipation may be related to abnormal intestinal neurotransmitter and aquaporin.Yougui Pills may be used to treat functional constipation by regulating the levels of SP,NO,5-HT,VIP and AQP3.

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