To observe the change of brain functional connective network in patients with primary dysmenorrhea by the moxibustion at heat-sensitized Guanyuan(RN 4)，based on the resting state functional magnetic resonance imaging(Rs-fMRI)，so as to provide potential imageological evidence for exploring the central mechanism of the moxibustion at heat-sensitized acupoints.Methods:A total of 60 patients with primary dysmenorrhea admitted in the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine from December 2016 to Febuary 2018 were selected as research objects.According to the test method of moxibustion sensation for heat-sensitized acupoints，and whether the heat-sensitive phenomenon occurred at Guangyuan(RN 4)，they were divided into heat-sensitized acupoint group(HA group，n=30)and non heat-sensitized acupoint group(NHA group，n=30).Rs-fMRI data collection was performed before and after the moxibustion in the 2 groups.DPARSFA software and an analysis method of brain functional connection(taking prefrontal cortex(PFC)as the seed point)were applied for data post-processing.Results:Compared with before the moxibustion:After the moxibustion in the HA group，regions related to enhanced PFC brain functional connection were the right brain，cerebral white matter area，putamen，lenticular nucleus，parietal lobe and precuneus.After the moxibustion in the NHA group，regions related to the enhanced PFC brain functional connection were the right brain，putamen and lenticular nucleus.And regions related to weakened PFC brain functional connection were the frontal lobe，anterior central gyrus and cerebral gray matter area.Compared with the NHA group:Before the moxibustion in the HA group，regions related to the weakened PFC brain functional connection were the left brain，frontal lobe，inferior frontal gyrus，left parietal lobe and inferior parietal lobe.After the moxibustion in the HA group，regions related to the enhanced PFC brain functional connection were the left brain stem and the left cerebellum.Regions related to weakened PFC brain functional connection were the left brain，cerebral white matter area，insula lobe and frontal lobe.Conclusion:The moxibustion at heat-sensitized Guanyuan(RN 4)can make the patients with primary dysmenorrhea change the brain functional connective network.The emergence of the sense of heat-sensitive moxibustion may be related to functions like enhancing the contact between the left brain stem and the left cerebellum，inhibiting the functional connection of the left brain-cerebral white matter area-insular lobe-frontal lobe，and indirectly affecting other limbic system related brain areas.