To test the concentration of ginsenoside in Shenfu Injection by high performance liquid chromatography(HPLC).Methods:The Agilengt-1100 HLPC was used in this study.ZORBAX SB-C18 column of the Agilent was selected as the chromatographic column，whose specific parameter was 5 μm × 250 mm × 46 mm，the column temperature was 30 ℃ and the sample volume was 10 μL.The condition of selecting the mobile phase was to study at the 1 mL/min velocity in the A flow phase water and the B flow phase acetonitrile.The rigor of the HPLC methodology was tested respectively.The scientificity of the method was gradually established by steps such as specificity test，precision test，stability test，repeatability test，linear regression test，sample recovery test and so on.Then the HPLC was used to perform specific detection and statistical analysis of the concentrations of ginsenoside Rg1，ginsenoside Re，ginsenoside Rb1 and ginsenoside Rd in the Shenfu Injection.Results: 1)The specificity outcome showed that the separation degrees of ginsenoside Rg1，ginsenoside Re，ginsenoside Rb1 and ginsenoside Rd were good.Their tailing factors were within the range of 098-104.The results showed that the HPLC method in the study of detecting the concentration of ginsenoside in the Shenfu Injection was of good specificity. 2)Linear regression equation：ginsenoside Rg1's was Y=11 278X-5471; ginsenoside Re's was Y=3 633X-3634; ginsenoside Rb1's was Y=8 640X-418; and ginsenoside Rd's was Y=7 088X+3503.All the correlation coefficients of the ginsenosides were higher than 0999，and the results showed that the linear relationship of the ginsenoside was good. 3)The RSD of peak area of ginsenoside Rg1，Re，Rb1 and Rd were 123%，128%，133%，120% and 135% respectively.The results showed that the HPLC instrument had good precision.The RSD of peak area of ginsenoside Rg1，Re，Rb1 and Rd were 044%，075%，025% and 085% respectively.The test results showed that the sample solution of Shenfu Injection was stable within 24 hours.The RSD of ginsenoside Rg1，Re，Rb1 and Rd were 118%，147%，186%，159% and 130% respectively.The results showed that the HPLC method had good repeatability. 4)The HPLC was used for determining the sample recovery rate，and the results showed that the RSD of ginsenoside Rg1，Re，Rb1 and Rd were 182%，104%，154% and 207% respectively，which showed that the sample recovery rate was good. 5)The concentrations of ginsenoside Rg1，Re，Rb1 and Rd in different batches of Shenfu Injection were quite different.Conclusion:The concentration of ginsenoside determined by HPLC in Shenfu Injection of different batches is quite different.