To establish the HPLC fingerprint of Heqi Powder in different batches，and to determine the contents of liquiritin，isoflavones glucoside，hyperoside，nuciferine，fibroblasts，aurantio-obtusin by QAMS.Methods:HPLC method was used to establish the fingerprint of 10 batches of Heqi Powder and the relative correction factors of the remaining five components with hyperoside as the reference.The contents were determined and the difference between the QAMS method and ESM method were compared.Results:HPLC fingerprint was established to calibrate a total of 28 common peaks.The RCF of hyperoside and liquiritin，isoflavones glucoside，nuciferine，fibroblasts，aurantio-obtusin were 152，071，043，071，056，RSD<30%.There was no significant difference between the measured values of QAMS method and ESM method.Conclusion:The fingerprint combined with QAMS can be used as a reference for evaluating the quality of Heqi Powder.