世界中医药

目的:观察五花茶发热模型鼠的退热效应,进一步明确其退热机制,同时研究其对肝肾功能的影响。方法:将50只SPF级SD大鼠纳入研究,随机分为空白组、模型组及五花茶组。其中模型组及五花茶组利用对干酵母制备发热模型,随后五花茶组大鼠接受高、中、低剂量灌胃［剂量分别为6480、3240、1620 g/(kg·d)］,空白组及模型组接受等体积生理盐水进行灌胃,各组均连续干预5 d,观察各组大鼠成模后不同时间点大鼠体温情况、干预前后大鼠肝肾功能的变化以及外周血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、IL-1β、环氧化酶1(COX-1)表达水平变化。结果:1)经过造模后大鼠体温明显升高,与空白组比较，差异有统计学意义(P<005),且体温呈现出双峰热型,分别在术后2 h和5 h时出现,在研究过程中部分造模大鼠出现明显的精神萎靡、毛色暗沉,甚至出现寒战症状,这提示本此造模成功。与模型组比较,五花茶组大鼠体温明显下降(P<005),且各五花茶干预组之间退热效应差异无统计学意义(P>005)。2)与空白组比较,模型组与五花茶组ALT、AST、BUN、Cr差异无统计学意义(P>005),在五花茶干预组中,五花茶+黄芪组与五花茶+大枣组小肠推进率水平低于五花茶组,差异有统计学意义(P<005),五花茶+大枣+黄芪组小肠推进率低于其余2组患者,差异有统计学意义(P<005)。3)与空白组比较,模型组及五花茶组大鼠IL-6、TNF-α及IL-1β均明显升高(P<005),其中五花茶组上述指标的改变较模型组明显,其中五花茶+大枣+黄芪组改变幅度最大(P<005)。4)Western blotting实验显示:与空白组比较,模型组及五花茶组大鼠下丘脑组织COX-1明显下降升高(P<005),其中五花茶组COX-1浓度有所上调,各组间COX-1的OD值差异无统计学意义(P>005)。结论:五花茶有明显退热效应,具有一定的剂量依赖性,且无肝肾毒性,临床可推广应用。

English Summary:

To observe the antipyretic effect of Wuhua tea fever model rats,to further clarify its antipyretic mechanism,and to study its effect on liver and kidney function.Methods:A total of 50 SPF SD rats were randomly divided into blank group,model group and tea group.The febrile model was made by dry yeast in the model group and the Wuhua tea group,and then the rats in the Wuhua tea group were given high,middle and low doses ［6480 g/(kg·d),3240 g/(mol·d)and 1620 g/(mol·d)］,respectively.The blank group and the model group received the same volume of normal saline for gavage.Each group was treated with continuous intervention for 5 days.The body temperature,liver and kidney function,peripheral serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),IL-1 β,TNF-α,TNF-β,TNF-α,TNF-β in peripheral serum were observed.The expression level of cyclooxygenase 1(COX-1)changed.Results:1)After modeling,the body temperature of the rats was significantly higher than that of the blank group(P<005),and the body temperature showed bimodal heat type,which appeared at 2 h and 5 h after operation,respectively.In the course of the study,some of the rats showed obvious mental malaise,dark hair color,and even shivering symptoms,which indicated that the model was successful.Compared with the model group,the body temperature of rats in the Wuhua tea group decreased significantly(P<005),and there was no significant difference in antipyretic effect among the intervention groups(P>005)2)Compared with the blank group,there was no significant difference in ALT,AST,BUN and Cr between the model group and the five-flower tea group(P>005).In the five-flower tea dry preparation group,the intestinal propulsive rate in the five-flower tea+astragalus membranaceus group and the five-flower tea+jujube group was lower than that in the five-flower tea group.The difference was statistically significant(P<005).The intestinal propulsive rate of Wuhua tea+jujube+astragalus membranaceus group was lower than that of the other two groups,the difference was statistically significant(P<005)3)Compared with the blank group,IL-6,TNF-α and IL-1 β in the model group and the tea group were significantly increased(P<005),and the changes of the above indexes in the tea group were more obvious than those in the model group.The change range of Wuhua tea+jujube+astragalus membranaceus group was the largest(P<005)4)Western blotting test showed that compared with the blank group,the COX-1 in the hypothalamus of the model group and the tea group decreased significantly(P<005),and the concentration of COX-1 in the group of the five flowers tea increased significantly(P<005),and the concentration of MDA in the hypothalamus of the model group and the tea group increased significantly(P<005).There was no significant difference in OD value of COX-1 among the three groups(P>005).Conclusion:Wuhua tea has obvious antipyretic effect in a dose-dependent manner and has no hepatorenal toxicity.it can be popularized and applied in clinic.

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