To observe the effect of Peiyuan Guben Decoction on inhibiting tumor growth in mice with Lewis lung cancer and prolonging the survival time in mice with H1299 lung cancer.Methods:A total of 100 C57BL/6 mice were randomly divided into a normal group，a Lewis lung cancer control group，a Lewis lung cancer plus cisplatin group，a Lewis lung cancer plus high-dose group，a Lewis lung cancer plus medium-dose group，a Lewis lung cancer plus low-dose group，a H1299 lung cancer control group，a H1299 lung cancer plus high-dose group，a H1299 lung cancer plus medium-dose group，and a H1299 lung cancer plus low-dose group，with 10 mice in each group.The normal group，control group and cisplatin group were continuously given 02 mL per day of normal saline for 15 days，and the treatment groups were respectively intragastric administrated 02 mL per day of Peiyuan Guben Decoction at the doses of 4496 g/mL，2248 g/mL and 1124 g/mL.After the trial，the immune organ weight，liver and kidney function，tumor weight and subcutaneous tumor volume were measured and the inhibition rate against tumor in mice with Lewis lung cancer was calculated.Meanwhile，the survival rate was calculated and the survival graph was drawn in mice with H1299 lung cancer.Results:Compared with the control group，the thymus index and spleen index of the high-and medium-dose groups had not significant differences(P>005)，and the thymus index of the low-dose group was significantly increased(P<005).The cisplatin group could significantly decrease the thymus index(P<001)and spleen index(P<005).The liver and kidney functions of Peiyuan Guben Decoction were normal and the cisplatin group was abnormal，and there were significant differences between them(P<001).The high-and medium-dose groups could reduce the tumor weight and the subcutaneous tumor volume compared with the control group(P<005)，and the tumor inhibition rate was respectively 50% and 42%.The cisplatin group could reduce the tumor weight and the subcutaneous tumor volume，and the tumor inhibition rate was 73%，which was statistically significant compared with the control group and the treatment groups(P<005).Peiyuan Guben Decoction could prolong the survival time，and the life extension rates of the high-，medium-and low-dose groups were respectively 47%，553% and 45%.Conclusion:Peiyuan Guben Decoction does not have hepatorenal toxicity and is safe.The high dose and medium dose of Peiyuan Guben Decoction could inhibit tumor growth，improve immunity function and prolong the survival time in mice with lung cancer.