世界中医药
文章摘要
引用本文:陈智昌,潘春梅,郭虹.桂枝茯苓丸对子宫肌瘤模型大鼠的作用机制[J].世界中医药,2019,(08):.  
桂枝茯苓丸对子宫肌瘤模型大鼠的作用机制
Discussion on Action Mechanism of Guizhi Fuling Pills on Uterine Fibroids in Rats
投稿时间:2019-02-21  
DOI:10.3969/j.issn.1673-7202.2019.08.018
中文关键词:  子宫肌瘤模型  大鼠  桂枝茯苓丸  病理变化  PI3K-AKT  信号通路
English Keywords:Uterine fibroids model  Rats  Guizhi Fuling Pills  Pathological changes  PI3K-AKT  Signaling pathway
基金项目:2014年度山东省科学基金项目(2014ZRB1461)
作者单位
陈智昌,潘春梅,郭虹 青岛市立医院中医科青岛266011 
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中文摘要:
      目的:评价桂枝茯苓丸对子宫肌瘤模型大鼠的疗效,同时观察其对子宫组织病理及PI3K-AKT信号通路的作用。方法:选取90只雌性SD大鼠并随机分为空白组、模型组及中药组,每组30只。模型组及中药组大鼠进行苯甲酸雌二醇注射液腹腔注射模拟子宫肌瘤模型,空白组大鼠接受等剂量生理盐水腹腔注射,造模成功后中药组大鼠进行桂枝茯苓丸灌胃,空白组及模型组大鼠接受等剂量生理盐水灌胃,1次/d,连续灌胃18 d。比较各组大鼠子宫湿重、子宫系数、子宫平滑肌层厚度变化,用Masson染色比对各组大鼠子宫组织病理的变化,用Western blotting检测子宫组织PI3K、AKT、p-AKT水平的变化,免疫组织化学法检测各组雌激素受体(ER)、孕激素受体(PR)的表达。结果:与空白组比较,模型组及中药组的子宫湿重、子宫系数及子宫平滑肌层厚度明显增加,中药组上述指标明显低于模型组,差异有统计学意义(P<005);Masson染色显示空白对照组子宫组织切片着色为红色,极少量染色纤维组织;模型组大鼠子宫环行肌和纵行肌可见大量的深蓝色纤维组织,甚至部分肌层组出现明显的团块状纤维结构;中药组大鼠子宫组织的纤维化程度明显受到抑制。与空白组比较,模型组及中药组大鼠子宫组织的PI3K、p-AKT水平上调,T-AKT各组水平差异无统计学意义(P>005),其中中药组PI3K、p-AKT水平较模型组明显降低,差异有统计学意义(P<005)。与空白组比较,模型组及中药组子宫平滑肌ER及PR阳性表达率升高,其中中药组低于模型组,差异有统计学意义(P<005)。结论:桂枝茯苓丸可明显抑制子宫肌瘤的发生发展,其作用机制可能是通过PI3K-AKT信号通路介导孕激素/雌激素受体实现的。
English Summary:
      To evaluate the effects of Guizhi Fuling Pills(GZFP)on uterine fibroids in rats,and to observe its effect on uterine histopathology and PI3K-AKT signaling pathway.Methods:A total of 90 female SD rats were randomly divided into a blank group,a model group and a Chinese medicine group,with 30 rats in each group.Rats in the model group and the Chinese medicine group were intraperitoneally injected with estradiol benzoate to simulate uterine leiomyoma model.Rats in blank group were injected intraperitoneally with the same dose of normal saline.Rats in the Chinese medicine group were given GZFP intragastric administration after successful modeling.Rats in the blank group and the model group were given the same dose of normal saline intragastric administration once a day,for continuous 18 d.The changes of uterine wet weight,uterine coefficient and thickness of uterine smooth muscle layer were compared in each group.The pathological changes of uterine tissues were compared by Masson staining.The concentrations of PI3K,AKT and p-AKT in uterine tissues were detected by Western blotting.The estrogen receptor(ER)and progesterone receptor(PR)were detected by immunohistochemistry.Results: 1)Compared with the blank group,the wet weight of uterus,the coefficient of uterus and the thickness of smooth muscle layer of uterus in the model group and the Chinese medicine group were significantly increased,and the above indexes in the Chinese medicine group were significantly lower than those in the model group.The difference was statistically significant(P<005). 2)Masson staining showed that in the blank control group,uterus tissue slices were red and few.In the model group,a large number of dark blue fibrous tissue could be seen in the uterine circular and longitudinal muscles,and even some of the myometrial groups had obvious lumpy fibrous structure. 3)Compared with the blank group,the concentrations of PI3K and p-AKT in the uterus of the model group and the Chinese medicine group were up-regulated.There was no significant difference in the concentrations of T-AKT among the three groups(P>005).The concentrations of PI3K and p-AKT in the Chinese medicine group were significantly lower than those in the model group(P<005). 4)Compared with the blank group,the positive expression rate of ER and PR in uterine smooth muscle of the model group and the Chinese medicine group increased,and the expression rate of ER and PR in the Chinese medicine group was lower than that in the model group.The difference was statistically significant(P<005).Conclusion:GZFP can significantly inhibit the occurrence and development of uterine leiomyoma,and its mechanism may be mediated by PI3K-AKT signaling pathway progesterone/estrogen receptor.
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