To investigate the effects of drug pair of “Ramulus Uncariae Cum Uncis and Rhizoma Acori Tatarinowii” on neurotransmitters and behavioral activities in TS rats.Methods:A total of 32 male SD rats were randomly divided into a normal group，a model group，an experimental group and a tabilium group，with 8 rats in each group.Intragastric administration of drugs was given when model establishing.At the same time，the normal group and the model group were given distilled water，and the other groups were given corresponding drugs，once per day，for 4 weeks.The changes of neurotransmitter content in each group of rats were observed，and the stereotyped behaviors，motor behaviors and autonomous activity times of each group of rats were evaluated on the 7th day of modeling and the 14th，21st and 28th day of instillation.Results:Compared with the normal group，there were no significant changes in dopamine and D2 receptors in the model group(P>005).Compared with the model group，the levels of dopamine and dopamine D2 receptor in the tabilium group，the experimental group all decreased(P<005).There was no significant difference between the experimental group and the tabilie group(P>005).By comparing the scores of stereotyped behaviors of TS rats in each group，after 21 days of treatment，the reduction of stereotyped behaviors of the experimental group was better than that of the tabilie group and the model group(P<005).By comparing the scores of exercise behaviors of TS rats in each group，there was no statistical significance between the tabilium group and the experimental group(P>005).The activity frequency of rats in the experimental group and the tabilium group decreased compared with the model group，and both of them had therapeutic effect.Conclusion:Drug pair of “Ramulus Uncariae Cum Uncis and Rhizoma Acori Tatarinowii” can inhibit the hypersensitivity of dopamine receptor and promote the metabolism of dopamine，thus inhibiting the stereotypical behavior of TS rats and reducing the activity frequency of TS rats.