To explore the effects of Guizhi Fuling Decoction on T lymphocyte subsets and serum tumor markers in patients with ovarian cancer after operation.Methods:A total of 88 patients with ovarian cancer after operation were enrolled in Hainan Tumor Hospital from March 2017 to March 2018.They were randomly divided into a control group(44 cases)and an observation group(44 cases)according to random number table.The control group received cytoreductive surgery combined with paclitaxel and carboplatin chemotherapy for 6 cycles.The observation group was treated with Guizhi Fuling Decoction for 6 cycles.Tumor markers：human epididymal secretory protein(HE4)，carbohydrate antigen 125(CA125)，carbohydrate antigen 199(CA199)，T lymphocyte subsets and immunoglobulin content platelet(PLT)，prothrombin time(PT)，partial thromboplastin time(APTT)，thrombin time(TT)，fibrinogen(FIB)，whole blood high and low cutting viscosity，SF-36 life quality score changes were observed before and after treatment.In the course of treatment，adverse reactions were monitored，including gastrointestinal reactions，liver and kidney function damage，bone marrow suppression，rash，etc.The curative effects were compared after treatment.Results:After treatment，CA125，CA199 and HE4 in the 2 groups were significantly lower than those before treatment(P<005)，and the above indexes in the observation group were significantly lower than those in the control group(P<005). 2)There was no significant difference in PLT，PT，APTT，TT，FIB，whole blood high cutting viscosity and whole blood low cutting viscosity before and after treatment in the control group(P>005).PLT，FIB，whole blood high cutting viscosity and whole blood low cutting viscosity in the observation group decreased significantly after treatment，while PT，APTT and TT increased significantly.PT，APTT and TT were significantly higher than those in the control group，PLT，FIB and whole blood low cutting viscosity were significantly higher than those before treatment.The high cutting viscosity and low cutting viscosity of whole blood were significantly lower than those of the control group(P<005). 3)After treatment，the scores of physiological function，physiological function，social function，mental health，emotional function，physical pain and vitality of the 2 groups were significantly increased than those before treatment(P<005)，and the scores of each dimension of the observation group were significantly higher than those of the control group(P<005). 4)After treatment，the levels of CD3+,CD4+,CD4+/CD8+，IgA and IgM in the 2 groups were significantly increased than those before treatment，and the levels of CD8+ in the observation group were significantly lower than those in the control group(P<005)，and the rest were significantly higher than those in the control group(P<005). 5)The rate of adverse drug reactions in the observation group was significantly lower than that in the control group(P<005).Conclusion:Guizhi Fuling Decoction can improve T lymphocyte subsets in peripheral blood of patients with ovarian cancer after operation，improve immunity，inhibit tumor markers and improve hypercoagulable state，thereby improving curative effect，improving quality of life with low incidence of adverse reactions.