世界中医药

基金项目:国家重点研发计划项目(2017YFC1701603，2017YFC1700701);财政部中央本级专项(2060302);科技基础性工作专项(2013FY114500);现代农业产业体系专项(CARS-21);国家中医药管理局委托项目(GZY-KJS-2018-004);国家自然科学基金青年基金项目(81603241)

作者	单位
赵子熙1，杨健1，李静1，张小波1，金艳1，郭见强2，郭兰萍1	1 中国中医科学院中药资源中心/道地药材国家重点实验室培育基地,北京,100700 2 山东华方药业有限公司,济南,250000

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中文摘要:

目的:建立超高效液相色谱三重四级杆串联质谱法同时测定不同来源乳香中7种乳香酸类成分的方法,比较不同来源乳香中该类成分的含量差异。方法:采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.8 μm);以0.1%甲酸-水(A)-0.1%甲酸-乙腈(B)溶液为流动相,梯度洗脱,体积流量为0.5 mL/min,柱温40 ℃;采用电喷雾离子源,正离子检测方式,得到相应的提取离子流图,以峰面积进行定量。结果:乳香中α-乳香酸、β-乳香酸、3-乙酰基-α-乳香酸、3-乙酰基-β-乳香酸、11-羰基-β-乙酰乳香酸、11-羰基-β-乙酰乳香酸和3-酮基-11α-羟基-β-乳香酸具有良好的线性关系,相关系数r均大于0.998 5,平均加样回收率为96.8%～102.0%,RSD均<3.5%。结论:该方法操作简便、准确、重复性好,为乳香中乳香酸类成分的深入研究及其质量控制提供了参考依据。

English Summary:

To establish a UPLC-MS/MS method for the simultaneous determination of 7 kinds of boswellic acids in Olibanum，and compare the contents in samples from different origins.Methods:The determination was performed on the ACQUITY UPLC BEH C18(2.1 mm × 100 mm，1.8 μm); the 0.1% formic acid-water(A)and 0.1% formic acid-acetonitrile(B)in gradient elution as mobile phase.The flow rate was 0.5 mL/min.The column temperature was 40 ℃.MS instrument was equipped with ESI+ ion source.Gaining the extracted ion chromatograms，then the peak area was used for quantitative.Results:The 7 kinds of boswellic acids：α-boswellic acid，β-boswellic acid，3-acetyl-α-boswellic acid，3-acetyl-β-boswellic acid，11-keto-β-boswellic acid，acetyl-11-keto-β-boswellic acid and 3-keto-11α-hydroxy-β-boswellic acid had good linearity，r>0.9985.The recovery rate was 96.8%～102.0%，with RSD<3.5%.Conclusion:The method is simple，accurate and repeatable，and provides a reference for in-depth research and quality control of boswellic acids in mastic.

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