| 引用本文:张文静，孙亚克.消渴痹通胶囊对高糖诱导的RSC96雪旺细胞损伤的保护作用及其机制[J].世界中医药,2019,(11):. |
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| 消渴痹通胶囊对高糖诱导的RSC96雪旺细胞损伤的保护作用及其机制 |
| Protective Effects of Xiaoke Bitong Capsules on High Glucose-Induced Injury of RSC96 Schwann Cells and Its Mechanism |
| 投稿时间：2019-05-31 |
| DOI：10.3969/j.issn.1673-7202.2019.11.013 |
| 中文关键词: 消渴痹通胶囊 高糖 RSC96雪旺细胞 损伤 保护作用 机制 NGF AKT |
| English Keywords:Xiaoke Bitong Capsules High glucose RSC96 Schwann cell Injury Protective effects Mechanism NGF AKT |
| 基金项目:国家自然科学基金项目(81070641) |
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| 中文摘要: |
| 目的:探讨消渴痹通胶囊对高糖诱导的RSC96雪旺细胞损伤的保护作用及其机制。方法:将RSC96雪旺细胞复苏及培养,采用免疫细胞化学法检测RSC96细胞的特异性蛋白S-100的表达情况,进行雪旺细胞鉴定;以高糖诱导造成RSC96雪旺细胞损伤,梯度高糖浓度比较,确定后续研究所采用的高糖浓度;通过MTT法检测消渴痹痛胶囊对高糖诱导的RSC96雪旺细胞的保护作用,采用免疫组织化学检测NGF蛋白水平和蛋白质印迹法检测P-AKT/AKT通路及caspase-3的蛋白水平。结果:1)RSC96雪旺细胞存活率随着消渴痹痛胶囊药物浓度的升高而增加(P<0.05)。2)NGF蛋白表达量随着消渴痹痛胶囊药物浓度增加(P<0.05)。3)p-AKT/t-AKT和caspase-3蛋白表达下调,且随着消渴痹痛胶囊药物浓度增加而减少(P<0.05)。结论:消渴痹痛胶囊可能通过抑制PI3K/AKT信号转导通路的激活,从而下调caspase-3蛋白的表达水平,抑制雪旺细胞的细胞凋亡,同时又上调NGF的表达水平,为周围神经的髓鞘细胞提供神经营养物质,从而对高糖诱导的RSC96雪旺细胞起到保护作用。 |
| English Summary: |
| To investigate the protective effects of Xiaoke Bitong Capsules on RSC96 Schwann cell injury induced by high glucose and its mechanism.Methods:RSC96 Schwann cells were resuscitated and cultured.The expression of specific protein S100 in RSC96 cells was detected by immunocytochemistry and identified by Schwann cells.The damage of RSC96 Schwann cells induced by high glucose was compared with the gradient high glucose concentration，and the high glucose concentration used in the follow-up study was determined.The protective effects of Xiaoke Bitong Capsule on RSC96 Schwann cells induced by high glucose was detected by MTT method.The level of NGF protein was detected by immunohistochemistry and the protein level of P-AKT/AKT pathway and caspase-3 was detected by Western blot.Results:1)The survival rate of RSC96 Schwann cells increased with the increase of Xiaoke Bitong Capsules concentration.2)The expression of NGF protein increased with the concentration of Xiaoke Bitong Capsules.3)The expression of p-AKT/t-AKT and caspase-3 protein was down-regulated and decreased with the increase of the concentration of Xiaoke Bitong Capsules(P<0.05).Conclusion:Xiaoke Bitong Capsules may down-regulate the expression of caspase-3 protein，inhibit the apoptosis of Schwann cells and up-regulate the expression of NGF by inhibiting the activation of PI3K/AKT signal transduction pathway.The myelin sheath cells of peripheral nerves are provided with neuronutrients，which can protect RSC96 Schwann cells induced by high glucose. |
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