世界中医药

目的:探讨加味身痛逐瘀汤对骨质疏松模型大鼠疗效,同时观察其对生物力学及骨代谢水平的影响。方法:将80只雌性wistar大鼠纳入研究,随机分为空白组(n=10)及造模组(n=70),所有造模组大鼠接受切除子宫模拟骨质疏松模型。将60只造模成功大鼠随机分为安慰组、对照组、中药低剂量组、中药中剂量组、中药高剂量组,每组15只。其中中药组大鼠接受加味身痛逐瘀汤灌胃,高剂量组(1.32 g/mL)、中剂量组(0.66 g/mL)、低剂量组(0.33 g/mL),灌胃1次/d,连续灌胃3周;安慰组大鼠接受生理盐水灌胃,10 mL/次,1次/d,连续灌胃3周;对照组大鼠给予降钙素注射液注射,2次/周,连续注射3周。3周后检测各组右侧股骨大鼠骨生物力学相关数据、右侧胫骨骨矿物密度和骨小梁结构变化、血清骨钙素、血清I型胶原氨基端肽原(PINP)浓度变化。结果:1)与空白组比较,造模组大鼠弹性模量、最大载荷降低(P<0.05),与安慰组比较,对照组及中药组弹性模量、最大载荷显著升高(P<0.01)。随着剂量的增加,中药组大鼠弹性模量、最大载荷显著增高(P<0.05)。2)与空白组比较,造模组BMD、BV/TV、Tb.N、Tb.Th降低(P<0.05);与安慰组比较,对照组及中药组骨密度显著升高(P<0.01);加味身痛逐瘀汤中剂量组和高剂量组大鼠骨密度较低剂量组增高(P<0.05)。3)与空白组比较,造模组血清BGP和PINP均明显升高,差异有统计学意义(P<0.05)。与造模组比较,中药组血清BGP和PINP均明显降低,随着加味身痛逐瘀汤浓度增加BGP和PINP下降的趋势更明显,差异有统计学意义(P<0.05)。结论:加味身痛逐瘀汤可能通过降低骨质疏松模型大鼠血清BGP和PINP含量,提高骨生物力学性能,纠正骨退行性改变,实现治疗骨质疏松的目的。

English Summary:

To investigate the effects of Jiawei Shentong Zhuyu Decoction on osteoporosis model rats，and to observe its effects on biomechanics and bone metabolism.Methods:A total of 80 female Wistar rats were randomly divided into a blank group(10 rats)and a model group(70 rats).All rats in model group received hysterectomy to simulate osteoporosis.60 successfully modeling rats were randomly divided into a placebo group，a control group，a low-dose group，a medium-dose group and a high-dose group，with 15 rats in each group.The rats in the traditional Chinese medicine group were given modified Shentong Zhuyu Decoction by gavage，with the high-dose group(1.32 g/mL)，the medium-dose group(0.66 g/mL)and the low-dose group(0.33 g/mL)，once a day for 3 weeks; the rats in the placebo group were given saline by gavage，10 mL/time，once a day for 3 weeks; the rats in the control group were given calcitonin injection，twice a week，for 3 weeks.After 3 weeks，bone biomechanical data，mineral density and trabecular structure of right tibia，serum osteocalcin and serum type I collagen telopeptide(PINP)were measured.Results:Compared with the blank group，the modulus of elasticity and the maximum load of the model group were decreased(P<0.05).Compared with the placebo group，the modulus of elasticity and the maximum load of the control group and the traditional Chinese medicine group increased significantly(P<0.01).With the increase of dose，the elastic modulus and maximum load of the rats in the traditional Chinese medicine group increased significantly(P<0.05).2)Compared with the blank group，BMD，BV/TV，Tb.N and Tb.Th in the model group decreased(P<0.05); BMD in the control group and the traditional Chinese medicine group increased significantly(P<0.01); BMD in the middle-dose group and the high-dose group increased significantly(P<0.05).3)Compared with the blank group，the serum BGP and P INP levels in the model group were significantly increased，and the difference was statistically significant(P<0.05).Compared with the model group，the serum BGP and P INP in the traditional Chinese medicine group were significantly decreased.With the concentration of modified Shentong Zhuyu Decoction increasing，the trend of BGP and PINP declining was more obvious，and the difference was statistically significant(P<0.05).Conclusion:Jiawei Shentong Zhuyu Decoction can improve the biomechanical properties of bone and correct the degenerative changes of bone by reducing the content of BGP and PINP in serum of osteoporosis model rats，so as to achieve the goal of treating osteoporosis.

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