世界中医药

目的：通过超高效液相色谱联合质谱法测定杜仲的3种成分及其药代动力学研究。方法：本研究的质谱采用电喷雾电离源(ESI)，通过多反应离子监测这一扫描方式对京尼平苷酸、绿原酸和松脂醇二葡萄糖苷等3种成分及内标用于定量分析的监测离子。结果：1）杜仲中京尼平苷酸、绿原酸、松脂醇二葡萄糖苷等3个指标性成分的分离良好，无内源性物质干扰，具有较好的专属性。2）UPLC方法严谨性，其精密度、稳定性试验、重复性试验、加样回收率等均良好。3）京尼平苷酸和松脂醇二葡萄糖苷在大鼠体内的药代动力学具有非线性特征，仅绿原酸存在线性特征；而CLz、Vz参数指标的差异比较大，显示大鼠血浆中杜仲提取物中的3种成分在大鼠体内的药代动力学代谢过程不一致。结论：UPLC-MS/MS的操作简单，结果具有较好的精密度、稳定性和重复性，可作为杜仲有效成分及药代动力学研究的可靠方法。

English Summary:

To determine the 3 components of Cortex Eucommiae by ultra-high performance liquid chromatography combined with mass spectrometry and their pharmacokinetics.Methods:Electrospray ionization source(ESI)was used to monitor the monitoring ions of geniposide chlorogenic acid and terpineol diglucoside，and internal standards for quantitative analysis of geniposide chlorogenic acid and terpineol diglucoside.Results:1)The 3 index components of Cortex Eucommiae such as geniposide，chlorogenic acid and terpineol diglucoside，were well separated and had no interference of endogenous substances，with good specificity.2)The UPLC method was rigorous，and its precision，stability test，repeatability test and sample recovery rate were all good.3)The pharmacokinetics of geniposide and turpentine diglucoside in rats was nonlinear，but only chlorogenic acid was linear.However，there were significant differences in the 2 parameters of CLz and Vz，indicating that the pharmacokinetic metabolism of the 3 components of Cortex Eucommiae extract in rat plasma was inconsistent.Conclusion:The operation of UPLC-MS/MS is simple，and the results have good precision，stability and reproducibility.It can be used as a reliable method for the study of effective components and pharmacokinetics of Cortex Eucommiae.

查看全文查看/发表评论下载PDF阅读器