| 引用本文:王景武1，李彬彬1，孙克陆1，吴永祥2.脑心通对高血压大鼠左心室重构的影响及机制[J].世界中医药,2019,(12):. |
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| 脑心通对高血压大鼠左心室重构的影响及机制 |
| Effects and Mechanism of Naoxintong on Left Ventricular Remodeling in Hypertensive Rats |
| 投稿时间：2019-09-27 |
| DOI：10.3969/j.issn.1673-7202.2019.12.021 |
| 中文关键词: 高血压 脑心通 血压 心肌损伤 过氧化物酶体增殖物激活受体 |
| English Keywords:Hypertension Naoxintong Blood pressure Myocardial injury Peroxisome proliferator-activated receptor |
| 基金项目:江苏省科技厅社会发展项目(BL2014088) |
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| 中文摘要: |
| 目的：探讨脑心通对自发性高血压（SHR）大鼠左心室重构的影响及机制。方法：将SHR大鼠随机分为低/高剂量组、模型组和对照组，每组20只，15只WKY大鼠为正常组。模型组灌胃30.0 mg/kg卡托普利，低/高剂量组灌胃1.0、2.0 g/kg脑心通，正常组、模型组大鼠则灌胃等量生理盐水，1次/d，各组均干预3个月。分别于干预4、8、12周检测各组大鼠尾动脉收缩压，超声心动图评估各组大鼠左心室形态及左心收缩功能，用苏木精-伊红（HE）染色观察大鼠心肌组织病理学形态，蛋白免疫印迹法检测各组大鼠心肌组织中PPARα、PPARγ蛋白表达。结果：与正常组比较，术后4周、8周、12周模型组尾动脉收缩压升高；而低/高剂量组和对照组较模型组降低，且高剂量组低于低剂量组，差异有统计学意义（P<0.05）。模型组LVDd、LVDs较正常组升高，而低/高剂量观察组和对照组较模型组降低，差异有统计学意义（P<0.05）；模型组LVSd、LVSs、LVEF、FS较正常组降低，而低/高剂量组和对照组较模型组升高，差异有统计学意义（P<0.05）。与正常组比较，模型组大鼠心肌组织中PPARα、PPARγ蛋白水平升高，差异有统计学意义（P<0.05），而对照组及低/高剂量组较模型组降低，差异有统计学意义（P<0.05）。结论：脑心通可剂量依赖性降低SHR大鼠血压，减轻大鼠左心室重构，其机制可能与上调PPARα、PPARγ蛋白表达相关。 |
| English Summary: |
| To investigate the effects and mechanism of Naoxintong on left ventricular remodeling in spontaneous hypertension(SHR)rats.Methods:SHR rats were randomly divided into a low/high dose experimental group, a model group and a control group, with 20 rats in each group and 15 WKY rats in the normal group.The model group was given 30.0 mg/kg captopril, and the low/high dose experimental group was given 1.0 and 2.0 g/kg Naoxintong, and the model group and the normal group were given the same amount of saline once a day.All rats were intervened for 3 months.Systolic blood pressure of tail artery was measured at 4, 8 and 12 weeks after intervention.Left ventricular morphology and left ventricular systolic function were evaluated by echocardiography.Myocardial histopathological morphology was observed by hematoxylin-eosin(HE)staining.PPARα and PPARγ protein in myocardium were detected by Western blotting(WB).Results:Compared with the normal group, the systolic pressure of tail artery in the model group increased at 4, 8 and 12 weeks after operation(P<0.05); compared with the model group, the systolic pressure of tail artery in the low/high dose experimental group and the control group decreased at 4, 8 and 12 weeks after intervention, and the systolic pressure of tail artery in the high dose experimental group was lower than that in the low dose experimental group(P<0.05).LVDd and LVDs in model group were higher than those in normal group, while those in low/high dose group and control group were lower than those in model group(P<0.05); LVSd, LVSs, LVEF and FS in the model group were lower than those in normal group, while those in the low/high dose group and the control group were higher than those in the model group(P<0.05).Compared with the normal group, the contents of PPARα and PPARγ protein in the myocardium of the model group increased(P<0.05).Compared with the model group, the contents of PPARα and PPARγ protein in the myocardium of the control group and the low/high dose experimental group decreased significantly(P<0.05).Conclusion:Naoxintong can reduce blood pressure and left ventricular remodeling in SHR rats in a dose-dependent manner.Its mechanism may be related to up-regulation of PPARα and PPARγ protein expression. |
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