世界中医药

目的：探讨白藜芦醇（Res）联合奥沙利铂（Oxa）对人结肠癌细胞恶性生物学行为的抑制作用及机制。方法：将体外培养结肠癌HCT-116细胞分为Res组、Oxa组、联合组及对照组，分别以100 μmoL/L Res、2 μmoL/LOxa、100 μmoL/L Res+2 μmoL/LOxa、等量生理盐水处理，各组分别处理24、36、48 h。采用噻唑蓝（MTT）法检测结肠癌HCT-116细胞增殖抑制情况；采用Transwell小室实验检测结肠癌HCT-116细胞侵袭能力；Wound Healing法检测结肠癌HCT-116细胞迁移能力；采用蛋白免疫印迹法检测结肠癌HCT-116细胞波形蛋白（Vimentin）蛋白表达情况。结果：干预48 h时，Res组、Oxa组及联合组结肠癌HCT-116细胞增殖抑制率分别为（28.41±6.25）%、（36.11±8.47）%、（83.61±12.72）%；干预48 h时，对照组、Res组、Oxa组及联合组结肠癌HCT-116细胞侵袭率分别（97.14±2.81）%、（81.23±4.52）%、（69.84±3.97）%、（37.11±3.58）%；迁移率分别为（68.57±4.02）%、（46.11±3.13）%、（31.25±2.86）%、（18.79±2.21）%；Vimentin蛋白相对表达量分别为0.93±0.14、0.52±0.08、0.31±0.04、0.13±0.02。Res组、Oxa组及联合组结肠癌HCT-116细胞增殖抑制率呈逐渐升高趋势（P<0.05）；与对照组比较，Res组、Oxa组及联合组结肠癌HCT-116细胞侵袭率、迁移率和Vimentin蛋白相对表达量降低，且联合组显著低于Res组与Oxa组，差异有统计学意义（P<0.05）。结论：Res与Oxa联合使用具有显著的协同效应，可更有效抑制结肠癌HCT-116细胞增殖，并降低其侵袭和迁移能力，其机制可能与下调Vimentin蛋白表达有关。

English Summary:

To investigate the inhibitory effect and mechanism of resveratrol(Res)combined with oxaliplatin(Oxa)on the malignant biological behavior of human colon cancer cells.Methods:Colon cancer HCT-116 cells were cultured in vitro.The Res group, the Oxa group, the combination group and the control group were treated with 100 μmoL/L Res, 2 μmoL/L Oxa and 100 μmoL/L Res+2 μmoL/L Oxa, the same amount of saline, and each group was treated for 24, 36, 48 h.The inhibition of proliferation of colon cancer HCT-116 cells was detected by MTT assay; Transwell chamber assay was used to detect the invasion ability of colon cancer HCT-116 cells; Wound healing method was used to detect the migration ability of colon cancer HCT-116 cells; The expression of Vimentin protein(vimentin)in colon cancer HCT-116 cells was detected by Western blotting(Wb)method.Results:At 48 h after intervention, the proliferation inhibition rates of HCT-116 cells in the Res group, the Oxa group and the combination group were(28.41±6.25),(36.11±8.47)and(83.61±12.72)%, respectively; At 48 h after intervention, the invasive rates of HCT-116 cells in the control group, the Res group, the Oxa group and the combination group were(97.14±2.81),(81.23±4.52),(69.84±3.97),(37.11±3.58)%, respectively; The mobility rates were(68.57±4.02),(46.11±3.13),(31.25±2.86), and(18.79±2.21)%, respectively; The relative expression levels of Vimentin protein were(0.93±0.14),(0.52±0.08),(0.31±0.04), and(0.13±0.02), respectively.The proliferation inhibition rate of colon cancer HCT-116 cells in the Res group, the Oxa group and the combination group increased gradually(P<0.05); Compared with the control group, the invasion rate, mobility rate and Vimentin protein expression of HCT-116 cells in the Res group, the Oxa group and the combination group significantly decreased, and the combination group was significantly lower than the Res group and the Oxa group(all P<0.05).Conclusion:The combination of Res and Oxa has significant synergistic effect, which can inhibit the proliferation of colon cancer HCT-116 cells and reduce its invasion and migration rate, and its mechanism may be related to the down-regulation of Vimentin protein expression.

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