| 引用本文:林深常，李运景，赵伟国，唐蕾，刘锐锋，周俊.复方广金钱草合剂改善肾草酸钙结石大鼠肾功能的效果及其机制[J].世界中医药,2020,(03):. |
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| 复方广金钱草合剂改善肾草酸钙结石大鼠肾功能的效果及其机制 |
| Effects and Mechanism Analysis of Compound Herba Desmodii Mixture in Improving Renal Function in Rats with Calcium Oxalate Stone |
| 投稿时间：2019-03-07 |
| DOI：10.3969/j.issn.1673-7202.2020.03.019 |
| 中文关键词: 复方广金钱草合剂 肾结石 草酸钙 大鼠 肾功能 氧化应激 炎性反应 |
| English Keywords:Compound Herba Desmodii Mixture Kidney stone Calcium oxalate Rat Renal function Oxidative stress Inflammation |
| 基金项目:中山市医学科研基金项目（2013A020051） |
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| 中文摘要: |
| 目的：探讨复方广金钱草合剂改善肾草酸钙结石大鼠肾功能的效果及其机制。方法：将SPF级雄性SD大鼠40只随机分为观察组和对照组，每组20只。观察组采用L-羟脯氨酸注射法诱导创立肾草酸钙结石大鼠模型，并以复方广金钱草合剂灌胃干预28 d。分别于对照组入组后和观察组建模前、建模后1 d、建模后28 d收集尿液标本，进行尿量、尿蛋白、尿肌酐、尿钙等肾功能相关指标的检测。并在收集尿液标本的同时取2组尾静脉血2 mL，进行血肌酐、血尿素氮等肾功能指标的检测。观察组干预28 d均双肾标本，其中左肾制成10%匀浆进行丙二醛(MDA)、超氧化物歧化酶(SOD)、胱甘肽过氧化物酶(GSH-Px)水平检测，右肾组织切片并通过免疫组织化学检测骨桥蛋白(OPN)和转化生长因子-β(TGF-β)等炎性相关蛋白表达情况。结果：与对照组比较，观察组建模后1 d尿量降低，尿蛋白、尿肌酐、尿钙、血肌酐、血尿素氮水平升高，差异有统计学意义（P<0.05）。与同组建模前比较，观察组建模后1 d尿量降低，尿蛋白、尿肌酐、尿钙、血肌酐、血尿素氮水平升高，差异有统计学意义（P<0.05）。与同组建模后1 d比较，观察组建模后28 d尿量升高，尿蛋白、尿肌酐、尿钙、血肌酐、血尿素氮水平降低，差异有统计学意义（P<0.05）。与对照组比较，观察组肾组织MDA水平以及OPN、TGF-β阳性表达率升高，SOD、GSH-Px水平降低，差异有统计学意义（P<0.05）。结论:复方广金钱草合剂可改善肾草酸钙结石大鼠肾功能，而改善氧化应激和炎性反应可能为其作用机制。 |
| English Summary: |
| To investigate the effect and mechanism of Compound Herba Desmodii Mixture on renal function in rats with calcium oxalate calculi.Methods:A total of 40 male SPF SD rats were randomly divided into a study group and a control group, with 20 rats in each group.The model of calcium oxalate nephrolithiasis was induced by L-hydroxyproline injecting in the study group.The rats were treated with Compound Herba Desmodii Mixture for 28 days.Urine samples were collected from the control group and study group before modeling, 1 day after modeling and 28 days after modeling.The urine volume, urinary protein, urinary creatinine and urinary calcium were detected.At the same time of urine samples taking, 2 mL of caudal vein blood were collected from the 2 groups.Serum creatinine, blood urea nitrogen and other renal function indexes were detected.The left kidney was made into 10% homogenate to detect the contents of MDA, SOD and GSH-Px.The right kidney was sectioned and the expressions of osteopontin(0PN)and transforming growth factor-beta(TGF-beta)were detected by immunohistochemistry.Results:Compared with those of the control group, the urine volume of the study group one day after the establishment of the model decreased, while the urine protein, urine creatinine, urine calcium, serum creatinine and serum urea nitrogen levels of the study group one day after the establishment of the mode were increased(P<0.05).Compared with those of the same group before modeling, the urine volume of the study group 1 day after modeling decreased, while the urine protein, urine creatinine, urine calcium, serum creatinine and serum urea nitrogen levels of the study group 1 day after modeling increased(P<0.05).Compared with those of the same group one day after model was established, the urine volume of the study group 28 days after modeling increased, while the urine protein, urine creatinine, urine calcium, serum creatinine and serum urea nitrogen levels of the study group 28 days after modeling decreased(P<0.05).Compared with those of the control group, the content of MDA and the positive expression rate of 0PN and TGF-beta in renal tissue of the study group increased, while the content of SOD and GSH-Px of the study group decreased(P<0.05).Conclusion:Compound Herba Desmodii Mixture can improve renal function in rats with calcium oxalate calculi, and improvement of oxidative stress and inflammation may be its mechanism. |
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