To study genetic diversity of its germplasm resources and to provide theoretical bases for the resource variety identification and molecular breeding of G.lucidum.based on SSR markers of the whole genome of Ganoderma lucidum.Methods:A total of 26 G.lucidum strains from different regions were amplified to screen out polymorphic sites using 60 pairs of SSR primers.The genotyping was carried out by capillary electrophoresis and the genetic diversity among the strains was analyzed.Results:Among 60 primer pairs, 24 SSR loci with polymorphic bands were selected.A total of 269 polymorphic bands were amplified, with an average of 11.20 polymorphic bands per SSR locus; a total of 150 alleles (Ne) were detected with an average of 6.23 type of gens.The polymorphism information content (PIC) of the primers were ranged from 0.57 to 0.91, and the average was 0.81.Genetic identity and genetic distance variation range were 0.28-0.95 and 0.06-0.73, respectively.The results of cluster analysis can well reflect the genetic relationship between various strains and can be divided into 4 subgroups with genetic distance coefficient of 0.17.The strains materials from the same region were scattered in various branches, which did not show obvious regional characteristics, indicating that the genetic background of G.lucidum strains was complex and highly polymorphic.Conclusion:The 24 selected SSR loci can be used to analyze the genetic diversity of the G.lucidum strains, which provide the theoretical basis for the introduction and breeding of the best varieties of G.lucidum.