To analyze the BAHD acyltransferase (AT) gene sequence of medicinal plants, Glehnia littoralis from bioinformatics perspective.Methods:Glehnia littoralis seedlings was treated with methyl jasmonate (MeJA).The real-time fluorescent quantitative PCR (RT-qPCR) method was used to detect the gene expression.A GlAT gene sequence with high expression and up-regulated expression after MeJA treatment were screened from established transcriptome data of Glehnia littoralis.The cDNA sequence of the gene was cloned in full-length; the physical and chemical properties, secondary structure and tertiary structure of the protein were analyzed based on obtained amino acid sequence; and NJ phylogenetic tree based on AT proteins sequence was build using MEGA 6.0 software.Results:The full-length ORF of GlAT was 1 443 bp, encoding 480 amino acid residues.The relative molecular weight of the protein was 52 844.14, and the isoelectric point was 6.92.It was a hydrophilic protein and belonged to the transferase superfamily.GlAT protein of Glehnia littoralis was closely related to the AT protein of Daucus carota subsp.sativus.Conclusion:For the first time, the full-length ORF sequence of GlAT gene from G.littoralis was obtained and bioinformatics analysis was performed, which laid foundation for further research on the function and genetic regulatory mechanism of this gene.