| 引用本文:包勒朝鲁，那生桑.蒙药材胡德日奈都日吉德的质量标准初步研究[J].世界中医药,2020,(05):. |
|
| 蒙药材胡德日奈都日吉德的质量标准初步研究 |
| Primary Study on Quality Standard of Mongolian Crude Drug Huderinaidurijide |
| 投稿时间：2018-05-08 |
| DOI：10.3969/j.issn.1673-7202.2020.05.011 |
| 中文关键词: 蒙药 胡德日奈都日吉德 大戟二烯醇 总萜 薄层色谱 含量测定；紫外分光光度法 质量标准 |
| English Keywords:Mongolian medicine Huderinaidurijide Euphol Total terpenoids TLC Content determination UV spectrophotometry Quality standard |
| 基金项目:内蒙古自治区科技计划项目——鹿角等20种蒙药材一般炮制工艺及质量标准研究 |
|
| 摘要点击次数: 466 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：建立蒙药胡德日奈都日吉德的质量标准。方法：参照《中华人民共和国药典》（四部）2015年版相关方法，对胡德日奈都日吉德杂质、水分、灰分、浸出物进行检测；采用硅胶G薄层板，以大戟二烯醇为对照，以石油醚(60~90 ℃)-乙酸乙酯(10：1)为展开剂，用10%硫酸乙醇溶液显色，对胡德日奈都日吉德进行鉴别；采用紫外分光光度法，以大戟二烯醇为对照，在545 nm波长下对胡德日奈都日吉德的活性成分萜类化合物进行定量分析。结果:胡德日奈都日吉德三批样品杂质在0.69%～0.75%之间，平均值为0.72%，RSD为4.26%；水分含量在7.68%～7.74％之间，平均值为7.72%，RSD为0.03%；总灰分0.20%～0.22%之间，平均值为0.21%，RSD为2.86%；酸不溶性灰分在0.21%～0.23%之间，平均值为0.22%，RSD为4.82%；浸出物1.24~1.35之间，平均值为1.31%，RSD为4.64%。在供试品的色谱中，与对照品色谱相应的位置上，显示相同颜色的斑点。总萜在0.672 0~5.379 0 mg/L呈良好的线性关系，该方法平均回收率为97.48%，RSD为0.478%，3批样品总萜含量在25.67~35.94 mg/g之间，均含量为31.03 mg/g。结论:根据实验结果，制定蒙药胡德日奈都日吉德药材（按干燥品计算）中杂质、水分、总灰分、酸不溶性灰分分别不得过1%，10.0%，0.4%、0.4%；水溶性浸出物不得少于1.0%；总萜含量不得少于20 mg/g。本办法可用于蒙药胡德日奈都日吉德药材的质量控制。 |
| English Summary: |
| To establish quality standard of Mongolian crude drug Huderinaidurijide.Methods:According to relevant methods in Chinese Pharmacopoeia(2015 edition Ⅳ)，impurity，moisture，ash and extract of Huderinaidurijide were tested.A silica gel G plate was used.Euphol was taken as a control.Petroleum ether(60～90 ℃)-ethyl acetate(10:1)was used as developing solvent and 10% sulfuric acid ethanol solution was used for color development.Huderinaidurijide was performed identification.UV spectrophotometry was applied.Taking euphol as a control，active ingredients-terpenoids of Huderinaidurijide were quantitatively analyzed at 545 nm wavelength.Results:The impurity of 3 batches of sample Huderinaidurijide was 0.69%～0.75%，with an average of 0.72% and a RSD of 4.26%.The moisture content ranged from 7.68%～7.74％，with an average of 7.72% and a RSD of 0.03%.The total ash was between 0.20% and 0.22%，with an average of 0.21% and a RSD of 2.86%.The acid-insoluble ash ranged from 0.21%～0.23%，with an average of 0.22% and a RSD of 4.82%.The extract ranged from 1.24 to 1.35，with an average of 1.31% and a RSD of 4.64%.In the chromatogram of the test product，spots of the same color were displayed at positions corresponding to the chromatogram of the control product.The total terpenoids showed a good linear relationship between 0.6720 and 5.3790 mg/L.The average recovery rate of this method was 97.48% and the RSD was 0.478%.The content of total terpenoids in the 3 batches of samples ranged from 25.67 to 35.94 mg/g，with an average of 31.03 mg/g.Conclusion:According to the results，it is formulated that impurity，moisture，total ash，and acid-insoluble ash in Mongolian crude drug Huderinaidurijide(calculated on dried products)can not exceed 1%，10.0%，0.4% and 0.4%，respectively.Water-soluble extract is not less than 1.0%.The content of total terpenoids is not less than 20 mg/g.This method can be used for quality control of Mongolian crude drug Huderinaidurijide. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
