To observe effects of electroacupuncture at Baihui(GV 20)and Yintang(EX-HN3)on cognitive function after sevoflurane anesthesia in rats，and to detect level changes of Shh pathway marker protein in brain tissue.Methods:Thirty SPF SD rats were included in this study and randomly divided into blank group(n=10)，anesthesia group(n=10)and electroacupuncture group(n=10).The rats in the blank group were only subjected to simulated capture.The rats in the anesthesia group inhaled 3.0% sevoflurane for 30 min，then they were anesthetized with 1.5% sevoflurane for 3 h.On this basis，the electroacupuncture group was treated with electroacupuncture for 7 d.After the intervention，Morris water maze was used to detect changes of learning and memory ability in each group.HE staining was used to observe structural changes of hippocampal tissue in each group.Western blot and Real-Time PCR were used to detect level changes of Shh，Gli，PI3K，pAKT protein and mRNA in hippocampal tissue in each group.Results:The Morris water maze showed that total swimming distance and escape latency in the anesthesia group and electroacupuncture group were significantly longer than those in the blank group(P<0.05)，and those in the electroacupuncture group were significantly improved compared with those in the anesthesia group(P<0.05).2)HE staining showed that the morphology of hippocampal tissue was normal in the blank group，and the hippocampal tissue in the anesthesia group and electroacupuncture group showed obvious structural destruction，disordered arrangement of cells and obvious deformation and reduction of neuron cells.The morphological structure of hippocampus in the electroacupuncture group was better than that in the anesthesia group.3)Western blot and PT-PCR showed that the expressions of Shh，Gli，PI3K，p-AKT protein and mRNA in hippocampus of rats in the anesthesia group and electroacupuncture group were lower than those in the blank group(P<0.05)，and those in electroacupuncture group were better than those in the anesthesia group(P<0.05).Conclusion:Sevoflurane can have a certain damage effect on cognitive ability.Electroacupuncture at Baihui(GV 20)and Yintang(EX-HN3)can obviously improve cognitive ability，and its mechanism may be related to activation of Shh pathway.