世界中医药

作者	单位
马卉1，张润田1，李航2，黄敏1，陈广山1，刘洋3，曲天歌4，包海兰5，周琳1，赵艳1，段行武1，李玲玲1	1 北京中医药大学东直门医院皮肤科,北京,100700 2 北京大学第一医院皮肤性病科,北京,100034 3 首都医科大学附属北京世纪坛医院,北京,100038 4 北京中医药大学东方医院教育处,北京,100078 5 内蒙古自治区人民医院中西医结合科,呼和浩特,010017

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中文摘要:

目的:研究不同浓度龙血素A对瘢痕疙瘩成纤维细胞增殖、凋亡、迁移能力的影响以及对Col-I、Col-III、α-SMA、Bax、Caspase-3蛋白表达水平的影响。方法:1)不同浓度龙血素A作用于瘢痕疙瘩成纤维细胞,用CellTiter-Blue活力检测试验检测龙血素A对瘢痕疙瘩成纤维细胞增殖的影响。2)流式细胞术检测龙血素A对瘢痕疙瘩成纤维细胞凋亡的影响。3)划痕实验检测龙血素A对瘢痕疙瘩成纤维细胞迁移的影响。4)Western-Blot测定龙血素A对瘢痕疙瘩成纤维细胞Col-I、Col-III、α-SMA、Bax、Caspase-3蛋白水平的影响。结果:龙血素A可抑制瘢痕疙瘩成纤维细胞增殖、迁移并诱导其凋亡,同时减少COL-I、COL-III、α-SMA蛋白表达,并促进Bax、Caspase-3蛋白表达,并且均呈现剂量依赖性。结论:龙血素A可以调控瘢痕疙瘩成纤维细胞增殖与凋亡的平衡、减少细胞外基质沉积,从而可能对KD发挥一定的治疗作用。

English Summary:

To study the effects of different concentrations of Loureirin A on the proliferation,apoptosis and migration of keloid fibroblasts and the protein expression levels of Col-I、Col-III、α-SMA、Bax、Caspase-3. Methods:1) Different concentrations of L-Auxin A act on keloid fibroblasts. CellTiter-Blue vitality test was used to test the effects of L-Auxin A on keloid fibroblast proliferation. 2) Flow cytometry was used to detect the effects of L-Auxin A on keloid fibroblasts apoptosis. 3) Scratch test was used to detect the effects of borneol A on keloid fibroblast migration. 4) Proteins expression levels of Col-I、Col-III、α-SMA、Bax、Caspase-3 were detected by Western-Blot. Results:Loureirin A can inhibit the proliferation and migration of keloid fibroblasts,and induce their apoptosis,reduce the protein expression of Col-I、Col-III、α-SMA and promote the protein expression of Bax and caspase-3,and there is a dosage dependent relationship. Conclusion:Loureirin A can regulate the balance of keloid fibroblast proliferation and apoptosis,reduce the deposition of extracellular matrix,so it may play a certain therapeutic effects on keloid.

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