| 引用本文:许多，刘梅，杨波，刘静，刘子修，司梁宏，陆瑜.HPLC法测定冬虫夏草胶囊中腺苷的含量[J].世界中医药,2020,(06):. |
|
| HPLC法测定冬虫夏草胶囊中腺苷的含量 |
| Determination of Adenosine Content in Dongxia Chongcao Capsules by HPLC |
| 投稿时间：2019-03-07 |
| DOI：10.3969/j.issn.1673-7202.2020.06.004 |
| 中文关键词: 冬虫夏草胶囊 腺苷 高效液相色谱法 含量测定 质量评价 质量控制 核苷 腺嘌呤核苷酸 |
| English Keywords:Dongxia Chongcao Capsules Adenosine HPLC Content determination Quality evaluation Quality Control Nucleoside Adenine Nucleotide |
| 基金项目:2015军区医学科技创新课题项目(15MS074) |
|
| 摘要点击次数: 513 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的:建立院内制剂冬虫夏草胶囊中腺苷含量的测定方法,为提高该制剂的质量标准提供依据。方法:采用高效液相色谱法,色谱柱为Hibar-C18柱(4.6 mm×150 mm,5 μm);流动相为pH值6.5的磷酸盐缓冲液-甲醇(85:15);检测波长为260 nm;柱温30 ℃;流速为1.0 mL/min;进样量为10 μL。结果:腺苷在0.032 7~0.163 5 μg范围内,线性关系良好(R2=0.999 4);加样回收率为99.8%～101.5%;测定3个批次的冬虫夏草胶囊中腺苷的平均含量分别为0.91 mg/粒,0.92 mg/粒,0.93 mg/粒,相对标准偏差(RSD)分别为0.44%、1.15%、2.06%。结论:该方法简便,准确,重现性好,灵敏度高,可作为冬虫夏草胶囊质量控制指标;所测3批冬虫夏草胶囊中腺苷的含量相近,质量可靠,可用于临床治疗。 |
| English Summary: |
| To develop an HPLC method for the determination of adenosine in Dongxia Chongcao Capsules and provide a basis for improving the quality standards of the preparation. Methods:HPLC was used and the analytical column was Hibar-C18 (4.6 mm×150 mm,5 μm). The mobile phase was phosphate buffer pH6.5-methanol (85:15). The detection wavelength was 260 nm. The column temperature was 30 ℃. The flow was 1.0 mL/min. The injection volume is 10 μl. Results:Adenosine had a good linear relationship (R2=0.999 4) in the 0.032 7～0.163 5 μg range,and the sample recovery was 99.8%～101.5%. The average contents of adenosine in 3 batch slices of Chinese caterpillar fungus Capsules was 0.91 mg,0.92 mg,0.93 mg. The relative standard deviations (RSD) were 0.44%,1.15%,and 2.06%,respectively. Conclusion:The contents of adenosine in the 3 batches of Dongxia Chongcao Capsules are analogical. This method is simple,accurate and available for the quality control of Dongxia Chongcao Capsules. The 3 batches of Dongxia Chongcao Capsules have similar adenosine content and reliable quality,which can be used for clinical treatment. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
