To explore the effects of ganoderma polysaccharide on liver fat deposits and NLRP3 inflammatory corpuscle expression of mice with acute liver injury.Methods:A total of 48 healthy KM mice were randomly divided into a control group, a model group and a test group, with 16 cases were in each group.Mice in the model group and the test group were constructed an acute alcoholic liver injury mice model combined the chronic alcohol feeding with the acute alcohol lavage method, and mice in the test group were treated by the lavage method of feeding 150 mg/kg-1 ganoderma polysaccharide, once a day.The liver function and the lipid metabolism indexes of mice were detected by an automatic biochemical analyzer; the serum inflammatory factor contents were detected by an enzyme-linked immunosorbent assay (ELISA) method; the liver tissue pathology morphology of mice was observed by a hematoxylin and eosin (HE) staining; the liver tissue fat deposits of mice were observed by an oil red O staining; the NLRP3 inflammatory corpuscle expression of mice was detected by an western blot (WB) method.Results:The experimental results showed the relevant expressions of mice in every group including the serum alanine aminotransferase (ALT), serum glutamic-oxaloacetic transaminase (AST), serum interleukin-1β (IL-1β), the serum interleukin-6 (IL-6), and the tumor necrosis factor-α (TNF-α), as well as the oil red O staining scores and the liver tissue NLRP3 inflammatory corpuscle were the same.Comparing the model group with the control group, it could be seen that the relevant expressions of the serum AST, ALT, triacylglycerol, total cholesterol, free fatty acids, IL-1β, IL-6, TNF-α, and the oil red O staining scores, and the liver tissue NLRP3 inflammatory corpuscle in the model group increased; compared with the model group, the test group level was lower, and the difference was statistically significant (all P<0.05).Conclusion:Ganoderma polysaccharide can inhibit liver tissue NLRP3 inflammatory corpuscle protein expression, and the inflammatory response and liver fat deposits of acute alcoholic liver injury mice, so as to relieve the liver tissue damage and to restore the liver function effectively.