| 引用本文:焦伟，崔红梅，霍叶琳，王静雅.黄芩苷调控活化Toll样受体4/核因子-κB信号通路对复发性流产小鼠的影响[J].世界中医药,2020,(06):. |
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| 黄芩苷调控活化Toll样受体4/核因子-κB信号通路对复发性流产小鼠的影响 |
| Effects of Baicalin on the Activation of Toll Like Receptor 4/NF-κ B Signaling Pathway in Mice with Recurrent Abortion |
| 投稿时间：2020-02-05 |
| DOI：10.3969/j.issn.1673-7202.2020.06.007 |
| 中文关键词: 黄芩苷 反复自然流产 活化Toll样受体4/核因子-κB信号通路 活化Toll样受体4/核因子κB信号通路 |
| English Keywords:Baicalin Recurrent spontaneous abortion Activation of Toll like receptor 4/nuclear factor kB signaling pathway Activation of Toll like receptor 4/nuclear factor κB signaling pathway |
| 基金项目:河北省医学科学研究重点课题计划项目（20150150） |
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| 中文摘要: |
| 目的：探讨黄芩苷对复发性流产小鼠的影响及作用机制。方法：参照Clark经典模型构建复发性流产小鼠，并将成功构建好的小鼠随机分为模型组和实验组，每组10只；同时建立正常妊娠小鼠模型10只作为对照组。于妊娠第2天，实验组灌胃0.4 mL/mg黄芩苷，模型组和对照组灌胃等量0.9%氯化钠注射液，1次/d，连续干预7 d。酶联免疫吸附试验检测小鼠血清中激素水平及母胎界面中炎性反应递质水平；免疫组织化学检测小鼠子宫组织巨噬细胞分布情况；蛋白免疫印迹法检测各组小鼠子宫组织活化Toll样受体4（TLR4）、核因子-κB、髓样分化因子88（MyD88）蛋白表达情况。结果：对照组、模型组和实验组小鼠母胎界面中TNF-α/IL-4为(2.78±0.36)，(4.89±0.58)和(3.39±0.34)，血清中E2为（25.16±3.69），（17.56±4.52），（22.36±4.16）ng/L。小鼠子宫组织中分化抗原14（CD14）蛋白相对表达量分别为（9.36±2.34），（68.12±13.52），（33.46±2.85）%，子宫组织中TLR4蛋白表达量为(0.23±0.02)，(1.25±0.33)，(0.45±0.12)，核因子-κB蛋白表达量为(0.59±0.14)，(1.52±0.36)，(0.78±0.25)，MyD88蛋白表达量为(0.63±0.24)，(2.34±0.22)，(1.26±0.31)，差异有统计学意义（P<0.05）。结论：黄芩苷可提高复发性流产小鼠雌激素水平，降低母胎界面中炎性状态，与抑制子宫内膜巨噬细胞浸润，调控TLR4-核因子-κB信号通路活性有关。 |
| English Summary: |
| To investigate the effects and mechanism of Baicalin on recurrent abortion mice.Methods:Construction of recurrent abortion mouse was based on Clark's classic model, and the mice with recurrent abortion successfully constructed were randomly divided into a model group and a test group with 10 mouse in each group.Meanwhile, normal pregnant mice with 10 mouse were as control the group.On the second day of pregnancy, 0.4 mL/mg baicalin was given by gavage in the test group, and 0.9% NaCl was given by gavage in the model group and the control group once a day for 7 days.Enzyme linked immunosorbent assay were used to detect the levels of hormones in serum and content of inflammatory factors in maternal-fetal interface; immunohistochemistry was used to detect the distribution of macrophages in mouse uterus; Western blotting was used to detect the expression of activated toll like receptor 4 (TLR4), nuclear factorκ B (NF-κB) and myeloid differentiation factor 88 (MyD88) protein expression in mouse uterus.Results:TNF-α/IL-4 were (2.78±0.36), (4.89±0.58) and (3.39±0.34) in the control group, model group and test group, and E2 were (25.16±3.69), (17.56±4.52) and (22.36±4.16) ng/L in the serum.The relative expressions of CD14 protein in the mouse uterus were (9.36±2.34), (68.12±13.52) and (33.46±2.85), respectively.The expressions of TLR4 protein expressions were (0.23±0.02), (1.25±0.33) and (0.45±0.12).NF-κB protein expressions were (0.59±0.14), (1.52±0.36) and (0.78±0.25), MyD88 protein expressions were (0.63±0.24), (2.34±0.22) and (1.26±0.31), all of which were statistically significant (P<0.05).Conclusion:Baicalin can improve the estrogen level of mouse with recurrent abortion, reduce the inflammatory state of the maternal fetal interface.It is related to the inhibition of intimal macrophage infiltration and the regulation of TLR4-NFκB signaling pathway activity.. |
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