Needle-type chemical sensor is a kind of detection instrument based on needle-like object(acupuncture needle,stainless steel needle,tungsten wire,needle plastic and platinum wire,etc.),which can realize the micro invasive in vivo and real-time monitoring of some specific biochemical substances in animals.Based on the work of iatrochemistry sensor laboratory,the third level scientific research laboratory of the National Administration of Traditional Chinese medicine,and referring to the research of needle sensor in China,this paper introduced the basic principals,research,application progress,especially the applicaton in TCM research of needle-type chemical sensor detecting calcium ion,hydrogen ion,oxygen partial pressure,dopamine,5-hydroxytryptamine,acetylcholine,noradrenaline and adenosine,in the hope of promoting the research of needle type chemical sensor and provide technical support for acupuncture research of traditional Chinese medicine.