世界中医药

作者	单位
靳贝贝1，裴香萍1，梁惠珍1，李一婵1，孙振华1，门九章2	1 山西中医药大学中药学院，晋中，030619； 2 山西中医药大学中西医结合临床学院，晋中，030619

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中文摘要:

目的：建立门氏柴胡理中颗粒定性定量质量标准控制方法。方法：采用薄层色谱法对门氏柴胡理中颗粒制剂中柴胡、黄芩进行薄层鉴别，采用高效液相色谱法（HPLC）对柴胡理中颗粒中的黄芩苷进行含量测定,色谱柱为Diamonsil-C18色谱柱（200 mm×4.6 mm,5.0 μm），流动相为甲醇-0.1%醋酸水溶液进行梯度洗脱，流速为1.0 mL/min，检测波长为280 nm，柱温为25 ℃,进样量为10 μL.结果：柴胡、黄芩薄层色谱斑点分离清晰,阴性均无干扰；回归方程为y=2 907 186.561 0x+21 224.436 6（r=0.999 8），黄芩苷在0.12～1.20 μg范围内具有良好的线性关系。平均加样回收率为99.45%，RSD为2.48%。结论：建立的方法方便、可靠，可作为门氏柴胡理中颗粒的薄层鉴别和含量测定质量控制方法。

English Summary:

To establish a qualitative and quantitative quality standard control method for the Menshi Chaihu Lizhong Granules.Methods:The Bupleurum chinense and Radix Astragali were qualitatively identified by thin-layer chromatography by the Menshi Chaihu Lizhong Granules.The content of baicalin in Menshi Chaihu Lizhong was determined by high performance liquid chromatography.Diamonsil-C18 column(200 mm×4.6 mm,5.0 μm)was used.The mobile phase was gradient eluted with methanol-0.1% aqueous acetic acid,the flow rate is 1.0 mL/min,the detection wavelength is 280 nm,the column temperature is 25 ℃,and the injection volume is 10 μL.Results:The chromatographic spots of Bupleurum chinense and Radix Scutellariae were clearly separated and there was no interference in the negative; the regression equation was y=2 907 186.561 0x+21 224.436 6(r=0.999 8),and baicalin had a good linear relationship in the range of 0.12 μg-1.20 μg.The average recovery was 99.45% and the RSD was 2.48%.Conclusion:The established method is reliable and scientific,and can be used for thin-layer identification andqualitative and quantitative quality control of the Menshi Chaihu Lizhong Granules.

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