To explore the efficacy of acupuncture at Siguan acupoint in the treatment of migraine based on nuclear factor-κ B signal pathway (NF-κ B).Methods:A total of 114 patients with migraine treated in Qifu Hospital of Guangzhou University of Traditional Chinese Medicine from February 2018 to February 2019 were randomly divided into a control group (n = 57) and an observation group (n = 57).The control group was treated with routine Western medicine，and the observation group was treated with acupuncture at Siguan point for 4 weeks.The symptoms，clinical efficacy，NF-κ B signal pathway and related indexes were compared between the 2 groups.Results:After treatment，procalcitonin gene-related peptide (CGRP)，VAS score，duration，seizure frequency，NF-κ B，cyclooxygenase inhibitor (COX-2)，interleukin-1 β (IL-1 β)，nitric oxide synthase (iNOS)，tumor necrosis factor-α (TNF-α)，anterior cerebral artery (ACA)，middle cerebral artery (MCA)，posterior cerebral artery (PCA)，vertebral artery (VA)，and the mean blood flow velocity of basilar artery (BA) were lower than those before treatment (P<0.05).The levels of β-endorphin (β-EP) and 5-hydroxytryptamine (5-HT) were higher than those before treatment(P<0.05)，and the observation group was better than the control group (P<0.05).The total effective rate of the observation group was 87.72%，which was higher than that of the control group (71.93%).The difference was statistically significant (P<0.05).Conclusion:Acupuncture and moxibustion at Siguan point can improve the signal pathway of NF-kappa B and the blood flow velocity of cerebral artery，so as to improve the symptoms of Migraine.