| 引用本文:穆威杉1，于娟1，赵晴1，史萌萌1，金钺2，谢红波1，刘金欣1,2，石林春2.基于DNA条形码技术的中药材木通种子鉴定研究[J].世界中医药,2020,(09):. |
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| 基于DNA条形码技术的中药材木通种子鉴定研究 |
| Identification of the Seeds of Akebiae Caulis,a Chinese Medicinal Material by DNA Barcoding Technique |
| 投稿时间：2020-02-10 |
| DOI：10.3969/j.issn.1673-7202.2020.09.008 |
| 中文关键词: 木通 种子 DNA条形码 ITS2 psbA-trnH 鉴定 混伪品 序列分析 |
| English Keywords:Akebiae Caulis Seeds DNA barcoding ITS2 psbA-trnH Identification Adulterants Sequence analysis |
| 基金项目:国家自然科学基金项目（81703659）；河北省教育厅青年拔尖人才项目（BJ2016002）；河北省高校重点学科建设项目（冀教高［2013］4号） |
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| 中文摘要: |
| 目的：利用核糖体内部转录间隔区2（ITS2）及叶绿体psbA-trnH序列对中药材木通种子进行鉴定，建立其种子DNA条形码鉴定方法，保障中药材木通种子的准确性。方法：收集木通种子样品15份，通过提取DNA、聚合酶链式反应（PCR）扩增、双向测序，获取其ITS2及psbA-trnH序列。基于BLAST分析、邻接（NJ）系统发育树构建进行物种鉴定分析。结果：共获得4种类型ITS2序列，2种类型psbA-trnH序列。ITS2及psbA-trnH均可区分木通与川木通、关木通，但ITS2序列可以区分木通与三叶木通、白木通，psbA-trnH序列无法区分木通与三叶木通、白木通。结论：DNA条形码技术可用于木通种子及其易混伪品的鉴定。 |
| English Summary: |
| By internal transcribed spacer 2(ITS2)and plastid intergenic region psbA-trnH sequences,the Akebiae Caulis seeds would be identified and DNA barcoding technology has been established,to guarantee the species authenticity of Akebiae Caulis seeds,a Chinese medicinal material.Methods:A total of 15 samples of Akebiae Caulis seeds were collected from different areas.Their ITS2 and psbA-trnH sequences have been obtained after DNA extraction,polymerase chain reaction(PCR)and bi-directional sequencing.Species identification analysis was based on BLAST analysis and neighbor joining(NJ)phylogenetic tree method.Results:A total of 4 types of ITS2 sequences and 2 types of psbA-trnH sequences were obtained.ITS2 and psbA-trnH sequences can distinguish Akebiae Caulis and Clematidis Armandii Caulis,and Caulis Aristolochiae Manshuriensis.But the ITS2 sequence can distinguish Akebia quinata and Akebia trifoliata,Akebia trifoliata var.austrais.The psbA-trnH sequence cannot distinguish Akebia quinata and Akebia trifoliata,Akebia trifoliate var.austrais.Conclusion:DNA barcoding can be useful for origin identification of Akebiae Caulis seeds and its adulterants. |
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