To study the optimization of leaf callus induction in Gardenia jasminoides Ellis.Methods:The callus induction was studied using leaves as explant,MS and WPM as basic medium adding different hormone combinations(6-BA,NAA and IBA).Results:Gardenia leaves had a 100% of callus induction rate in WPM+6-BA 0.5 mg/L+NAA 0.5 mg/L or 6-BA1.0 mg/L+NAA 0.1(or 0.5)mg/L,while 70% and 74% were the best induction rates in MS+6-BA 1.0(or 1.5)mg/L+NAA 0.5 mg/L,respectively.The leaf callus of Gardenia did not induce cluster buds in the redifferentiation medium.Conclusion:Compared with MS medium,the callus was easy to be induced in WPM medium,and the redifferentiation of Gardenia callus was difficult.