| 引用本文:田亚1,2，徐志超2，胡开治3，王华磊1，宋经元2.栀子叶片愈伤组织诱导优化研究[J].世界中医药,2020,(09):. |
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| 栀子叶片愈伤组织诱导优化研究 |
| Optimization of Callus Induction in Leaves of Gardenia jasminoides |
| 投稿时间：2020-02-10 |
| DOI：10.3969/j.issn.1673-7202.2020.09.009 |
| 中文关键词: 栀子 叶片 愈伤组织诱导 MS培养基 WPM培养基 6-BA NAA IBA |
| English Keywords:Gardenia jasminoides Ellis leaves Callus induction MS medium WPM medium 6-BA NAA IBA |
| 基金项目:国家自然科学基金项目（81973424）；重庆市社会事业与民生保障科技创新专项（cstc2017shmsA130021） |
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| 中文摘要: |
| 目的：对栀子（Gardenia jasminoides Ellis）叶片愈伤组织诱导进行优化研究。方法：通过以叶片为外植体，MS和WPM为基本培养基，添加不同激素组合（6-BA、NAA和IBA）进行愈伤组织诱导研究。结果：栀子叶片在WPM+6-BA 0.5 mg/L+NAA 0.5 mg/L以及6-BA 1.0 mg/L+NAA 0.1（或0.5）mg/L中均有100%的诱导率；在MS培养基上，MS+6-BA 1.0（或1.5）mg/L+NAA 0.5 mg/L的诱导率最好，分别为70%和74%。栀子叶片愈伤组织在再分化培养基上均未诱导出丛生芽。结论：WPM培养基比MS培养基更易诱导栀子叶片愈伤组织，且栀子愈伤组织的再分化较困难。 |
| English Summary: |
| To study the optimization of leaf callus induction in Gardenia jasminoides Ellis.Methods:The callus induction was studied using leaves as explant,MS and WPM as basic medium adding different hormone combinations(6-BA,NAA and IBA).Results:Gardenia leaves had a 100% of callus induction rate in WPM+6-BA 0.5 mg/L+NAA 0.5 mg/L or 6-BA1.0 mg/L+NAA 0.1(or 0.5)mg/L,while 70% and 74% were the best induction rates in MS+6-BA 1.0(or 1.5)mg/L+NAA 0.5 mg/L,respectively.The leaf callus of Gardenia did not induce cluster buds in the redifferentiation medium.Conclusion:Compared with MS medium,the callus was easy to be induced in WPM medium,and the redifferentiation of Gardenia callus was difficult. |
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