| 引用本文:郭梦月1,2，姜汶君1,2，于景盛1,2，杨美华1，庞晓慧1,2.基于高通量测序技术研究药食两用薏苡仁中污染真菌多样性[J].世界中医药,2020,(09):. |
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| 基于高通量测序技术研究药食两用薏苡仁中污染真菌多样性 |
| Analyzing Fungal Diversity in Medicinal and Edible Coicis Semen using High-throughput Sequencing |
| 投稿时间：2020-02-10 |
| DOI：10.3969/j.issn.1673-7202.2020.09.010 |
| 中文关键词: 薏苡仁 真菌多样性 高通量测序 药食两用 ITS2 产毒真菌 真菌鉴定 镰刀菌属 |
| English Keywords:Coicis Semen Fungal diversity High-throughput sequencing Medicinal and edible ITS2 Mycotoxin-producing fungi Fungal identification Fusarium |
| 基金项目:中国医学科学院医学与健康科技创新工程项目（2017-I2M-1-013） |
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| 中文摘要: |
| 目的：调查药食两用薏苡仁中污染真菌多样性，为其安全使用提供参考依据。方法:收集薏苡仁样品18批，提取真菌DNA并扩增ITS2序列，基于Illumina MiSeq PE250平台进行高通量测序。结果：共检测到4门18纲44目99科149属的真菌，子囊菌门Ascomycota是最优势菌门，镰刀菌属Fusarium（3.05%～60.32%）是属水平最优势属，其次是曲霉属Aspergillus（2.20%～45.44%）、白僵菌属Beauveria（0.07%～63.21%）、链格孢属Alternaria（0.80%～11.92%）、Arachnomyces（0.03%～39.36%）和青霉属Penicillium（0.24%～8.03%）。此外，共检测到5种潜在产毒真菌，分别是烟曲霉A.fumigatus、土曲霉A.terreus、梨孢镰刀菌F.poae、囊状青霉P.capsulatum和展青霉P.paxilli。结论：高通量测序技术可以快速有效地检测薏苡仁中污染真菌种类，为薏苡仁污染真菌毒素提供风险预警。 |
| English Summary: |
| To investigate the fungal diversity in medicinal and edible Coicis Semen,thus providing evidence for its safe use.Methods:A total of 18 batches of Coicis Semen samples were collected.The total DNA was extracted,and the ITS2 sequences were amplified.Then,the amplicons were sequenced using the Illumina MiSeq PE250 platform.Results:The fungi detected were denominated to 4 phylum,18 class,44 orders,99 families,and 149 genera.Ascomycota was the dominant fungus at the phylum level.At the genus level,Fusarium was the most predominant,followed by Aspergillus,Beauveria,Alternaria,Arachnomyces and Penicillium,accounting for (3.05%～60.32%),(2.20%～45.44%),(0.07%～63.21%),(0.80%～11.92%),(0.03%～39.36%) and (0.24%～8.03%).In addition,a total of 5 potential mycotoxin-producing fungi were detected,namely,A.fumigatus,A.terreus,F.poae,P.capsulatum and P.paxillin.Conclusion:High-throughput sequencing technology can quickly and effectively detect fungal contamination in Coicis Semen,thereby providing early warning for mycotoxin contamination in Coicis Semen. |
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