世界中医药
文章摘要
引用本文:胡娅婷1,2,徐志超2,田亚2,高冉冉2,贺京3,辛天怡2,浦香东2,王瑀2,刘霞1,宋经元2.大田不同水分条件对蒙古黄芪生长及品质的影响[J].世界中医药,2020,(09):.  
大田不同水分条件对蒙古黄芪生长及品质的影响
Effects of Different Water Contents in the Field on the Growth and Quality of Astragalus membranaceus var.mongholicus
投稿时间:2020-02-10  
DOI:10.3969/j.issn.1673-7202.2020.09.011
中文关键词:  蒙古黄芪  干旱胁迫  生长  次生代谢物  毛蕊异黄酮  毛蕊异黄酮葡萄糖苷  品质  HPLC
English Keywords:Astragalus membranaceus var.mongholicus  Drought stress  Growth  Secondary metabolites  Calycosin-7-glucoside  Calycosin  Quality  HPLC
基金项目:中国医学科学院医学与健康科技创新工程本草基因组协同创新团队项目(2016-I2M-3-016)
作者单位
胡娅婷1,2,徐志超2,田亚2,高冉冉2,贺京3,辛天怡2,浦香东2,王瑀2,刘霞1,宋经元2 1 武汉理工大学化学化工与生命科学学院武汉430070 2 中国医学科学院北京协和医学院药用植物研究所国家中医药管理局中药资源保护重点研究室中药资源教育部工程研究中心北京100193 3 内蒙古盛齐堂生态药植有限公司呼和浩特011500 
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中文摘要:
      目的:探讨不同水分条件对蒙古黄芪生长发育及次生代谢物含量的影响,为研究蒙古黄芪品质提升及代谢调控机制奠定基础。方法:以蒙古黄芪为试验材料,开展大田试验,设置常规水分条件(CK)、轻度干旱胁迫(A1)、重度干旱胁迫(A2)3个不同处理,考察了不同水分胁迫下蒙古黄芪的株高、根长、商品根长、根鲜重等性状,并采用高效液相色谱法分析蒙古黄芪有效成分含量的变化规律。结果:轻度干旱胁迫时,黄芪株高较对照组降低7.60%、但根长提高11.23%,根鲜重提高26.07%,商品根长提高10.40%;在重度干旱胁迫下,株高、根长、商品根长、根鲜重较对照组均降低,分别为13.67%、12.60%、17.05%、5.5%;HPLC结果显示轻度干旱胁迫下毛蕊异黄酮葡萄糖苷含量比对照组高11.74%;重度干旱胁迫下毛蕊异黄酮葡萄糖苷含量比对照组低19.93%。而在干旱胁迫下毛蕊异黄酮含量与对照组差异不大。结论:轻度干旱胁迫能够有效促进黄芪幼苗生长并提高黄芪药材品质,为蒙古黄芪规范化种植技术提供参考。
English Summary:
      To explore the effects on the growth and development and secondary metabolite content of Astragalus membranaceus var.mongholicus under under different water contents,and to lay a foundation for quality improvement of Astragalus membranaceus var.mongholicus and metabolism regulation mechanism.Methods:By using Astragalus membranaceus var.mongholicus as the test material,the test was conducted in the field.A total of 3 different drought treatments[regular soil-water content (CK),mild drought stress (A1),and strong drought stress (A2)]were applied to investigate the plant height,root length,commercial root length,fresh root weight and other characteristics of Astragalus membranaceus var.mongholicus under above different water stress.And the High Performance Liquid Chromatography(HPLC)was used to analyze the law of change of active components in Astragalus membranaceus var.mongholicus.Results:Under mild drought stress,the plant height of Astragalus membranaceus var.mongholicus was significantly reduced by 7.60% compared with the control group.But the root length was significantly increased by 11.23% and the fresh root weight was significantly increased by 26.07%.The commercial root length was significantly increased by 10.40%; under severe drought stress,plant height,root length,commercial root length and root fresh weight were significantly reduced compared with the control group,which were 13.67%,12.60%,17.05% and 5.5%,respectively.HPLC results showed that,the content of Calycosin-7-glucoside was 11.74% higher than that of control group under mild drought stress,however,the content of Calycosin-7-glucoside was 19.93% lower than that of control group under severe drought stress.The content of calycosin was not significantly different from that of the control group under drought stress.Conclusion:Mild drought stress can effectively promote the growth of Astragalus membranaceus var.mongholicus seedlings growing and improve the quality of the Radix Astragali seu Hedysari medicinal materials,so as to provide reference for the standardized planting technology of Astragalus membranaceus var.mongholicus.
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