To establish a new PCR identification method based on COI sequence to quickly identify sika deer,Malurong Tablets and Xunlurong Tables.Methods:A total of 60 batches of Lurong Tablets from different sources were collected.By comparing the difference of COI gene sequences between fakes such as sika deer,red deer and reindeer,the identification primers Cne-F,Rt-F and Co-R of sika deer,red deer and reindeer velvet antler were designed according to the SNP identification location site.The main factors of polymerase chain reaction (PCR) results,such as denaturation time,annealing temperature,annealing time,and extension time,were investigated and optimized.Results:The two-site specific PCR identification system constructed in this study,based on the COI identification site,can obtain 294 bp sika deer and red deer specific fragments by PCR amplification,and produce 514 bp reindeer deer specific fragments,New Zealand red deer and other pseudo-pars and negative and control strip-free band.Conclusion:This experiment established a simple and reliable PCR identification method for other fake Cornu Cervi Pantotrichum such as sika deer,red deer and reindeer.