To quickly identify Rubus Chingii Hu，the medicinal material from different regions without damage using near-infrared diffuse reflectance spectrometry.Methods:The near-infrared diffuse reflectance spectrometry was used to detect the original samples directly without any chemical treatment to obtain the complete spectral information of the samples.Through the combination of cluster analysis，similarity calculation，and principal component analysis，multiple pattern recognition technologies，the samples were qualitatively analyzed to achieve the rapid and non-destructive identification of Rubus Chingii Hu from different regions.Results:Although the chemical components of Rubus Chingii Hu from different regions were different，the similar components showed extremely similar on the near-infrared spectrum.In the wavelength region of 1 401.4～2 252.5 nm，the pattern recognition method based on the cluster analysis could be used for rapid identification of Rubus Chingii Hu origin，which was consistent with the results of similarity calculation and principal component analysis.Conclusion:The method is accurate，reliable，fast and nondestructive，and with few samples.It provides a new method reference for the analysis and identification of the origin of traditional Chinese medicine，and has a good application prospect.