| 引用本文:王丹1，赵瑞珍1，李小黎1，赵芸2，赵子珺2，唐启盛1.益肾调气法对血管性痴呆模型大鼠脑单胺神经递质的干预作用[J].世界中医药,2020,(10):. |
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| 益肾调气法对血管性痴呆模型大鼠脑单胺神经递质的干预作用 |
| Intervention Effects of Tonifying Kidney and Regulating Qi Method on Cerebral Monoamine Neurotransmitters in Model Rats with Vascular Dementia |
| 投稿时间：2019-10-13 |
| DOI：10.3969/j.issn.1673-7202.2020.10.005 |
| 中文关键词: 血管性痴呆 颐脑解郁方 去甲肾上腺素 多巴胺 5-羟色胺 益肾调气法 动物实验 |
| English Keywords:Vascular dementia Yinao Jieyu Formula Norepinephrine Dopamine 5-hydroxytryptamine Tonifying kidney and regulating qi method Animal experiments |
| 基金项目:国家中医药行业专项（201507001-12）——基于云数据的中医药防治抑郁症动态临床疗效评价研究；中医药传承与创新“百千万”人才工程（岐黄工程）岐黄学者；北京中医药大学自主选题资助项目（2018-JYBZZ-JS164）；北京中医药大学自主选题资助项目（2019-JYB-XS-ZB03） |
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| 中文摘要: |
| 目的:通过探讨血管性痴呆（VD）大鼠脑内单胺神经递质变化，研究益肾调气法对VD大鼠的干预机制。方法:选购雄性Wistar大鼠，通过旷场试验分为正常组、卒中组。MCAO法制备缺血性脑卒中模型，常规饲养28 d后经水迷宫试验筛选VD大鼠，并随机分为观察组和模型组。正常组和模型组灌胃蒸馏水，观察组灌胃颐脑解郁方，干预4周。灌胃第1、2、4周对各组大鼠行水迷宫检测；第4周灌胃后取材，采用高效液相检测大鼠右侧海马、前额叶皮质5-HT、NE、DA含量。结果:与正常组比较，模型组大鼠1、2、4周跳台潜伏期缩短、错误次数增加、水迷宫潜伏期明显延长，差异有统计学意义（P<0.01）；与模型组比较，观察组第2、4周水迷宫潜伏期缩短，差异有统计学意义（P<0.01），第4周跳台潜伏期延长、错误次数减少，差异有统计学意义（P<0.01）。模型组皮质及海马NE、DA、5-HT含量较正常组降低，差异有统计学意义（P<0.01），观察组海马DA含量较模型组升高，差异有统计学意义（P<0.01）。结论：益肾调气法可改善VD大鼠学习记忆能力，其作用机制可能与调节大鼠脑内NE、DA及5-HT含量相关。 |
| English Summary: |
| To study the intervention mechanism of tonifying kidney and regulating qi method on vascular dementia(VD)rats by investigating the changes of monoamine neurotransmitters in the brain of VD rats.Methods:Male Wistar rats were selected and divided into a normal group and a stroke group by open field test.MCAO method was used to prepare ischemic stroke model.After 28 d of routine feeding，VD rats were screened by water maze test and randomly divided into a treatment group and a model group.The normal group and the model group were infused with distilled water，and the treatment group was infused with Yinao Jieyu Formula for 4 weeks.At the 1st，2nd and 4th weeks of gavage，the rats in each group were tested for water maze，and at the 4th week after gavage，the material was taken and the contents of 5-HT，NE，DA in the right hippocampus and prefrontal cortex of rats was detected by high-performance liquid.Results:Compared with the normal group，the latent period of 1，2 and 4 weeks of platform jumping were shortened，the number of errors increased，and the latent period of water maze was significantly prolonged(P<0.01).Compared with the model group，the latent period of water maze at 2 and 4 weeks of treatment group was shortened(P<0.01)，and the latent period of platform jumping at 4 weeks was prolonged and the number of errors decreased(P<0.01).The content of NE，DA，5-HT in the cortex and hippocampus of the model group was lower than that in the normal group(P<0.01)，and the hippocampal DA content in the treatment group was higher than that in the model group(P<0.01).Conclusion:The tonifying kidney and regulating qi treatment can improve the learning and memory ability of VD rats，and its mechanism may be related to the content of NE，DA and 5-HT in the rat brain. |
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