| 引用本文:艾福录，吕武，赵国华，林杰.蓝莓花青素对人胃腺癌BGC-823细胞增殖、侵袭的影响及机制研究[J].世界中医药,2020,(10):. |
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| 蓝莓花青素对人胃腺癌BGC-823细胞增殖、侵袭的影响及机制研究 |
| Effects and Mechanism Study on Blueberry Anthocyanins on Proliferation and Invasion of Human Gastric Adenocarcinoma BGC-823 Cells |
| 投稿时间：2020-03-27 |
| DOI：10.3969/j.issn.1673-7202.2020.10.009 |
| 中文关键词: 胃腺癌 蓝莓花青素 增殖 侵袭 P53 半胱氨酸蛋白酶-3 |
| English Keywords:Gastric adenocarcinoma Blueberry anthocyanins Proliferation Invasion P53 Cysteine protease-3 |
| 基金项目:辽宁省科学计划技术项目（2019-ZD-0580） |
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| 中文摘要: |
| 目的：探讨蓝莓花青素（BA）对人胃腺癌BGC-823细胞增殖、侵袭的影响及相关机制。方法：低、中、高剂量实验组与对照组人胃腺癌BGC-823细胞分别以25、50、100 μg/mL BA与等量生理盐水处理，分别处理12 h、24 h、48 h。采用噻唑蓝（MTT）与流式细胞仪检测人胃腺癌BGC-823细胞增殖与凋亡；采用Transwell小室实验与免疫细胞化学法检测人胃腺癌BGC-823细胞侵袭与P53、半胱氨酸蛋白酶-3（Caspase-3）蛋白表达。结果：干预48 h时，对照组、低、中、高剂量实验组人胃腺癌BGC-823细胞P53与Caspase-3蛋白阳性细胞百分比分别为（11.12±1.01）％，（24.31±2.18）％，（47.56±2.91）％，（66.49±5.14）%与（15.04±1.23）％，（28.22±3.09）％，（69.36±5.42）％，（86.52±6.15）%。实验组胃腺癌BGC-823细胞增殖抑制率随BA浓度增大而逐渐升高；与对照组比较，低、中、高剂量实验组人胃腺癌BGC-823细胞凋亡率、P53与Caspase-3蛋白阳性细胞百分比均显著升高，侵袭率降低，呈浓度依赖性（均P<0.05）。结论：BA可有效抑制胃腺癌BGC-823细胞增殖，诱导其凋亡，并降低侵袭能力，其机制可能与上调P53与Caspase-3蛋白表达有关。 |
| English Summary: |
| To investigate the effects and related mechanism of blueberry anthocyanins (BA) on the proliferation and invasion of human gastric adenocarcinoma BGC-823 cells.Methods:Human gastric adenocarcinoma BGC-823 cells exp-L/M/H and the control group were treated with BA at the concentration of 25，50，100 μg/mL and the same amount of normal saline respectively，and each group was treated for 12，24，and 48 hours.The proliferation and apoptosis of human gastric adenocarcinoma BGC-823 cells was detected by MTT assay and Flow cytometry; Transwell chamber assay and immunocytochemistry method were used to detect the invasion of human gastric adenocarcinoma BGC-823 cells and the expression of P53 and caspase-3 protein.Results:At 48 h after intervention，the percentage of P53 cells and Caspase-3 protein positive cells in human gastric adenocarcinoma BGC-823 cells in the exp-L/M/H were (11.12±1.01）％，(24.31±2.18）％，(47.56±2.91）％，(66.49±5.14)% and (15.04±1.23）％，(28.22±3.09）％，(69.36±5.42）％，and (86.52±6.15)%，respectively.With the increase of BA concentration，the proliferation inhibition rate of gastric adenocarcinoma BGC-823 cells in experimental group increased gradually; Compared with the control group，the apoptotic rate，P53 protein and Caspase-3 protein positive percentage of human gastric adenocarcinoma BGC-823 cells in the exp-L/M/H groups significantly increased，while the invasive rate decreased and the trend was concentration-dependent (all P<0.05).Conclusion:BA can effectively inhibit the proliferation of gastric adenocarcinoma BGC-823 cells，induce its apoptosis，and reduce its invasive ability，which may be related to the up-regulation of P53 protein and Caspase-3 protein expression. |
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