| 引用本文:郭婕1，潘彦舒1，庞大承1，牟天龙2.黄芩苷介导光动力疗法治疗宫颈癌的疗效及其机制研究[J].世界中医药,2020,(11):. |
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| 黄芩苷介导光动力疗法治疗宫颈癌的疗效及其机制研究 |
| Study on Therapeutic Effects and Mechanism of Baicalin-mediated Photodynamic Therapy for Cervical Cancer |
| 投稿时间：2020-02-01 |
| DOI：10.3969/j.issn.1673-7202.2020.11.013 |
| 中文关键词: 黄芩苷 姜黄素 光动力疗法 肿瘤抑制 凋亡机制 |
| English Keywords:Baicalin Curcumin Photodynamic therapy Tumor suppression Apoptosis mechanism |
| 基金项目:西藏自治区藏医药区域协同创新中心第一批培育项目（2017XTCX014）;2018年度北京中医药大学教育科学研究课题（2018-JYB-JS106）;2019年度北京中医药大学在读研究生课题（2019-JYB-XS-008） |
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| 中文摘要: |
| 目的：观察黄芩苷介导光动力疗法(PDT)治疗宫颈癌的疗效，探讨其可能机制。方法：将宫颈癌Hela细胞悬液注入裸鼠皮下，构建移植瘤模型，成模后随机均匀分为模型组、黄芩苷-PDT观察组、PDT观察组、姜黄素-PDT观察组4组，每组18只。PDT观察组：肿瘤周围注射100 μL生理盐水，6 h后瘤周注射硼砂缓冲液100 μL后行PDT治疗，照射剂量为80 J/cm2（435 nm波长，0.60 A，0.54 W，距离肿瘤表面3~5 cm，照射2 min 28 s）；黄芩苷-PDT观察组：以80 μg/mL的黄芩苷溶液100 μL代替生理盐水，其他同PDT观察组；姜黄素-PDT观察组：以50 μmol/L的姜黄素溶液100 μL代替生理盐水，其他同PDT观察组；模型组：以自然光照代替PDT治疗，其他同PDT观察组。治疗1 d后，各组处死6只取材肿瘤组织制备成病理切片，HE染色观察组织病理学改变；另外处死6只，利用蛋白质印迹法检测肿瘤组织中Bcl-2和Bax蛋白的表达情况；剩余6只分别于治疗第1、3、7、14 d测量肿瘤体积，计算肿瘤体积抑瘤率。结果：模型组肿瘤体积持续增大，光动力治疗1 d后肿瘤组织出现坏死，肿瘤生长受到抑制，黄芩苷-PDT观察组和姜黄素-PDT观察组肿瘤体积在3 d内均无增大趋势，随后逐渐增大。黄芩苷-PDT观察组Bcl-2和Bax蛋白表达与模型组比较，差异有统计学意义(P<0.05)。结论：黄芩苷介导的PDT能够有效抑制宫颈癌体外移植瘤的增长，其机制可能通过促进癌细胞凋亡发挥作用。 |
| English Summary: |
| To observe the therapeutic effects of baicalin-mediated photodynamic therapy (PDT) on cervical cancer and explore its possible mechanism. Methods:A cervical cancer Hela cell suspension was injected subcutaneously into nude mice to construct a transplanted tumor model. After the model was formed, it was randomly and evenly divided into 4 groups: a model group, a baicalin-PDT treatment group, a PDT treatment group, and a curcumin-PDT treatment group , with 18 cases in each group. PDT treatment group: 100μL of saline was injected around the tumor, and 100 μL of borax buffer was injected around the tumor 6 hours later, followed by PDT treatment. The irradiation dose was 80J/cm2 (435nm wavelength, 0.60A, 0.54W, 3-5cm from the tumor surface, irradiation 2 min28s); Baicalin-PDT treatment group: 100 μL of baicalin solution at 80 μg/mL instead of normal saline, others were the same as PDT treatment group; curcumin-PDT treatment group: 50 μmol/L curcumin solution instead of 100 ml normal saline, others were the same as PDT treatment group; model group: natural light instead of PDT treatment, others were the same as PDT treatment group. After 1 day of treatment, 6 rats were sacrificed in each group and their tumor tissues were prepared for pathological sections, and the histopathological changes were observed by HE staining; another 6 were sacrificed, and the expression of Bcl-2 and Bax proteins in tumor tissues were detected by Western blot technology; their tumor volume of 6 remaining rats, was measured only on the 1st, 3rd, 7th, and 14th days of treatment, and the tumor volume inhibition rate was also calculated. Results:The tumor volume in the model group continued to increase. Tumor tissue appeared necrosis and tumor growth was inhibited after 1 day of photodynamic therapy. The tumor volume of baicalin-PDT treatment group and curcumin-PDT treatment group did not increase within 3 days, and then gradually increased. The expression of Bcl-2 and Bax proteins in the baicalin-PDT treatment group was significantly different from that in the model group (P<0.01). Conclusion:Baicalin-mediated PDT can effectively inhibit the growth of cervical cancer xenografts in vitro, and its mechanism may play a role in promoting cancer cell apoptosis. |
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