| 引用本文:杨芊芊，高歌，王瀚泽，侯晓梦，白雪，陈素娟，聂静，李强，司银楚.牛膝促神经干细胞增殖的有效部位筛选[J].世界中医药,2020,(12):. |
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| 牛膝促神经干细胞增殖的有效部位筛选 |
| Screening of Effective Parts of Twotoothed Achyranthes Root a for Promoting Neural Stem Cells Proliferation |
| 投稿时间：2019-10-01 |
| DOI：10.3969/j.issn.1673-7202.2020.12.002 |
| 中文关键词: 牛膝 神经干细胞 增殖 活性部位筛选 |
| English Keywords:Twotoothed achyranthes root Neural stem cells Proliferation Bioactive part screening |
| 基金项目:国家自然科学基金面上项目(81373830)——基于神经干细胞巢调控的中药促进脑损伤修复的机制研究 |
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| 中文摘要: |
| 目的:研究牛膝不同提取分离部位对体外培养的神经干细胞增殖的作用。方法:采用系统溶剂法以及柱色谱法将牛膝分成不同部位,以CCK-8法检测牛膝各部位对体外培养的神经干细胞增殖的效果,并筛选出较好的作用部位和浓度。结果:牛膝正丁醇部位(25 μg/mL,P<0.01)和其多糖部位(12.5 μg/mL,P<0.01)对神经干细胞增殖活性有明显促进作用。结论:牛膝正丁醇部位和多糖部位可能是其促神经干细胞增殖的主要活性部位。 |
| English Summary: |
| To study the effects of different extracted parts of twotoothed achyranthes root on the proliferation of neural stem cells cultured in vitro. Methods:The systemic solvent method and column chromatography were used to separate the twotoothed achyranthes root into different parts. The effects of various parts of twotoothed achyranthes root on the proliferation of neural stem cells were detected by CCK-8 method, and the optimal parts and concentration was screened. Results:The n-butanol part(25 μg/mL,P<0.01)and the polysaccharide part(12.5 μg/mL, P<0.01)significantly promoted the proliferation of neural stem cells. Conclusion:The n-butanol and polysaccharide part of twotoothed achyranthes root may be the main part for promoting the proliferation of neural stem cells. |
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