Based on Smurf2 crosstalk TGF-β1/Smad signaling pathway to study the effect and mechanism of Qigui Fomula active components on transforming growth factor β1 (TGF-β1)-induced human embryo lung fibroblast MRC-5. Methods:MRC-5 was cultured with 10 ng/mL TGF-β1 in vitro, mRNA level of α-SMA was detected by PCR instrument to judge whether the model was successfully made. Normal control group, model group (10 ng/mL TGF-β1 treatment), prednisone treatment group, high-dose group, middle-dose group and low-dose group of effective components of Qigui Fomula were set, CCK-8 method was used to detect the proliferation of MRC-5, Western blot was used to detect the expression level of Smad7 and SnoN protein in each group, and RT-PCR was used to detect the mRNA level of Smurf2 in each group. Results:Compared with the normal control group, the 48 h model group had significant cell proliferation, and the mRNA level of α-SMA increased (P<0.01), and the modeling was successful. Compared with the normal control group, MRC-5 treated with 10 ng/mL TGF-β1 showed that Smad7 and SnoN protein levels were significantly reduced and Smurf2 protein mRNA levels were increased (P<0.01 or P<0.05). The cell proliferation of the Qigui Foluma group with different concentrations was reduced, which could reverse the changes of the above indicators and showed a certain dose-effect relationship. Conclusion:The effective components of Qigui Foluma can inhibit the proliferation of MRC-5 cells, and can reduce the ubiquitination of Smad7 and SnoN mediated by Smurf2 in MRC-5 cells, restore the expression of Smad7 and SnoN proteins, and thereby regulate the occurrence of pulmonary fibrosis development.