| 引用本文:彭艳芳，张莹雯，张亚兵，夏玉坤，陈巍.芪归方有效组分治疗特发性肺纤维化的实验研究[J].世界中医药,2020,(12):. |
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| 芪归方有效组分治疗特发性肺纤维化的实验研究 |
| Study on the Effective Components of Qigui Fomula in the Treatment of Idiopathic Pulmonary Fibrosis |
| 投稿时间：2020-04-25 |
| DOI：10.3969/j.issn.1673-7202.2020.12.009 |
| 中文关键词: 芪归方有效组分 Smad泛素化调节因子2 Smad蛋白 核转录共抑制因子 特发性肺纤维化 |
| English Keywords:Effective Component of Qigui Foluma Smad ubiquitination regulator 2 Smad protein Nuclear transcription co-suppressor Idiopathic pulmonary fibrosis |
| 基金项目:湖北省卫生计生委中医药科研基金面上项目（ZY2019M080）；第六批全国老中医药专家学术经验继承工作项目（国中医药人教发［2017］29号） |
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| 中文摘要: |
| 目的：基于Smurf2串话TGF-β1/Smad信号通路研究芪归方有效组分对转化生长因子β1(TGF-β1)诱导人胚肺成纤维细胞MRC-5的影响及机制。方法：体外予以10 ng/mL TGF-β1培养MRC-5细胞，PCR仪检测α-SMA的mRNA水平判断模型是否成功。设立正常对照组、模型组（10 ng/mL TGF-β1处理）、泼尼松处理组、芪归方有效组分高剂量组、中剂量组、低剂量组，采用CCK-8法检测MRC-5的增殖情况，Western blot检测各组细胞Smad7、SnoN蛋白的表达水平，RT-PCR检测各组细胞Smurf2的mRNA水平。结果：与正常对照组比较，48 h模型组细胞增殖明显，α-SMA的mRNA水平增加（P<0.01），造模成功。经10 ng/mL TGF-β1处理细胞MRC-5，与正常对照组比较，Smad7、SnoN蛋白水平显著降低，Smurf2蛋白mRNA水平升高（P<0.01或P<0.05）。不同浓度芪归方有效成分处理组细胞增殖降低，可逆转以上各指标的改变，呈一定的量效关系。结论：芪归方有效组分能抑制MRC-5细胞增殖，可通过减少MRC-5细胞中Smurf2所介导的Smad7、SnoN的泛素化，恢复Smad7、SnoN蛋白的表达，从而调节肺纤维化的发生发展。 |
| English Summary: |
| Based on Smurf2 crosstalk TGF-β1/Smad signaling pathway to study the effect and mechanism of Qigui Fomula active components on transforming growth factor β1 (TGF-β1)-induced human embryo lung fibroblast MRC-5. Methods:MRC-5 was cultured with 10 ng/mL TGF-β1 in vitro, mRNA level of α-SMA was detected by PCR instrument to judge whether the model was successfully made. Normal control group, model group (10 ng/mL TGF-β1 treatment), prednisone treatment group, high-dose group, middle-dose group and low-dose group of effective components of Qigui Fomula were set, CCK-8 method was used to detect the proliferation of MRC-5, Western blot was used to detect the expression level of Smad7 and SnoN protein in each group, and RT-PCR was used to detect the mRNA level of Smurf2 in each group. Results:Compared with the normal control group, the 48 h model group had significant cell proliferation, and the mRNA level of α-SMA increased (P<0.01), and the modeling was successful. Compared with the normal control group, MRC-5 treated with 10 ng/mL TGF-β1 showed that Smad7 and SnoN protein levels were significantly reduced and Smurf2 protein mRNA levels were increased (P<0.01 or P<0.05). The cell proliferation of the Qigui Foluma group with different concentrations was reduced, which could reverse the changes of the above indicators and showed a certain dose-effect relationship. Conclusion:The effective components of Qigui Foluma can inhibit the proliferation of MRC-5 cells, and can reduce the ubiquitination of Smad7 and SnoN mediated by Smurf2 in MRC-5 cells, restore the expression of Smad7 and SnoN proteins, and thereby regulate the occurrence of pulmonary fibrosis development. |
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