| 引用本文:赵向荣，牛广旭，张小辉，辛礼辉.益气养阴方对宫颈癌大鼠免疫功能及癌组织的影响[J].世界中医药,2020,(12):. |
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| 益气养阴方对宫颈癌大鼠免疫功能及癌组织的影响 |
| Effects of Yiqi Yangyin Formula on Immune Function and Cancer Tissues of Cervical Cancer Rats |
| 投稿时间：2020-03-27 |
| DOI：10.3969/j.issn.1673-7202.2020.12.013 |
| 中文关键词: 宫颈癌 益气养阴方 免疫功能 癌组织 抑瘤率 |
| English Keywords:Cervical cancer Yiqi Yangyin Formula Immune function Cancer tissue Tumor inhibition rate |
| 基金项目:河北省青年科技课题指令性项目（20170227）；邯郸市科技局课题（1123108081—8） |
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| 中文摘要: |
| 目的：探讨益气养阴方对宫颈癌大鼠免疫功能及癌组织P16、Ki67、P53、Survivin表达的影响。方法：采用右前腋下接种宫颈癌细胞U14的方法构建宫颈癌大鼠模型，将宫颈癌大鼠随机分为模型组、低剂量实验组和高剂量实验组，每组12只。低剂量实验组和高剂量实验组大鼠分别灌胃益气养阴方0.435、1.36 mL/kg/d，模型组灌胃等量生理盐水，连续干预30 d。采用流式细胞仪检测大鼠外周血免疫性T细胞亚群；采用HE染色与Wb法分别检测癌组织病理学变化与P16、Ki67、P53、Survivin蛋白表达。结果：与模型组比较，低剂量实验组和高剂量实验组大鼠外周血CD3+、CD4+、NKT细胞比例及癌组织P16、P53蛋白相对表达量均升高，高剂量实验组高于低剂量实验组；CD8+细胞比例、肿瘤重量与体积及癌组织Ki67、Survivin蛋白相对表达量均降低或缩小，高剂量实验组低于或小于低剂量实验组（P<0.05）；且高剂量实验组的抑瘤率高于低剂量实验组（P<0.05）。结论：益气养阴方可有效上调宫颈癌大鼠癌组织P16、P53蛋白表达，下调Ki67、Survivin蛋白表达，从而抑制肿瘤生长，同时改善免疫功能，发挥治疗宫颈癌的作用。 |
| English Summary: |
| To investigate the effects of Yiqi Yangyin Formula on the immune function and the expression of P16, Ki67, and P53, Survivin in cancer tissues of cervical cancer rats. Methods:The cervical cancer rat model was constructed by inoculating cervical cancer cells U14 in the right anterior armpit, and the cervical cancer rats were randomly divided into a model group and a low/high dose experimental group, each with 12 rats. Rats in the low/high-dose experimental group were given 0.435 and 1.36 mL/（kg·d） of Yiqi Yangyin Formula, respectively by gavage, and the model group was given the same amount of normal saline for 30 days. Flow cytometry was used to detect the peripheral blood immune T cell subsets in rats; HE staining and Wb method were used to detect the pathological changes of cancer tissues and the expression of P16, Ki67, P53, and Survivin protein, respectively. Results:Compared with the model group, the ratio of CD3+, CD4+, NKT cells and the relative expression of P16 and P53 proteins in the peripheral blood of rats in the low/high-dose experimental group were increased. The high-dose experimental group was higher than the low-dose experimental group; CD8+ cell ratio, tumor weight and volume, and the relative expression of Ki67 and Survivin protein in cancer tissues were reduced or shrunk. The high-dose experimental group was lower or lower than the low-dose experimental group(P<0.05); and the tumor suppression rate of the high-dose experimental group was higher than that of the low-dose experimental group(P<0.05). Conclusion:Yiqi Yangyin Formula can effectively up-regulate the expression of P16 and P53 protein in cervical cancer tissues, and down-regulate Ki67, Survivin protein expression, inhibit tumor growth, improve immune function, and play a role in treating cervical cancer. |
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