| 引用本文:姚璐莎，王雁芳，范伏元.通痹颗粒对类风湿关节炎继发肺间质病变模型大鼠的保护作用[J].世界中医药,2020,(12):. |
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| 通痹颗粒对类风湿关节炎继发肺间质病变模型大鼠的保护作用 |
| Protective Effect of Tongbi Granule on Rats with Pulmonary Interstitial Disease Secondary to Rheumatoid Arthritis |
| 投稿时间：2019-05-22 |
| DOI：10.3969/j.issn.1673-7202.2020.12.014 |
| 中文关键词: 类风湿关节炎 肺间质病变 SD大鼠 通痹颗粒 益气通络 炎性反应 病理变化 保护作用 |
| English Keywords:Rheumatoid arthritis Pulmonary interstitial lesion model SD Rats Tongbi Granules Benefiting qi and unblocking collterals Inflammatory response Pathological changes Protective effect |
| 基金项目:长沙市科学技术局2015年科技成果转化基金项目(K1058015-31) |
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| 中文摘要: |
| 目的:观察通痹颗粒对类风湿关节炎继发肺间质病变模型大鼠的保护作用,探讨通痹颗粒是否是通过阻断经典Wnt信号传导通路来多方位调节机体免疫系统从而保护肺脏。方法:60只SD大鼠随机分为通痹颗粒组、硫唑嘌呤组和模型组(MC)、正常组(NC),正常对照组除外,其余各组采用牛Ⅱ型胶原联合博莱霉素诱导的类风湿关节炎继发肺间质病变模型,分别于造模成功后7 d、14 d和28 d 3个时间点观察关节炎指数。28 d时处死大鼠,测定血清中TNF-α的含量、观察肺组织切片病理变化。结果:实验阶段通痹颗粒组大鼠关节炎指数评分由开始时的8.25分最终下降到5.12分,与模型组比较,3个观察时间点关节炎指数得分均低于模型组,差异均有统计学意义(均P<0.05);28 d时与模型组比较,通痹颗粒组大鼠血清TNF-α水平(122.59 pg/mL)低于模型组(179.87 pg/mL),差异有统计学意义(P<0.01),与正常组相当(P>0.05);HE及Masson染色观察RA-ILD大鼠肺间质组织形态学显示,模型组大鼠肺间质形态明显改变,通痹颗粒与正常组形态接近。结论:通痹颗粒对牛Ⅱ型胶原联合博莱霉素诱导的类风湿关节炎继发肺间质病变具有明显保护作用。 |
| English Summary: |
| To observe the protective effect of Tongbi Granule on rats with pulmonary interstitial disease secondary to rheumatoid arthritis, and to investigate whether Tongbi Granules protect the lungs by blocking the classical Wnt signaling pathway and regulating the body's immune system in multiple directions.Methods:A total of 60 SD rats were randomly divided into normal group(NC),Tongbi Granule group,Azathioprine group and model group (MC). Bovine type II collagen combined with Bleomycin were used in the other groups to induce pulmonary interstitial disease secondary to rheumatoid arthritis model ,except the normal control group .The arthritis index was observed at three time points:7 d,14 d and 28 d after successful modeling. At 28 days, the rats were sacrificed, the content of TNF-α in serum was measured, and the pathological changes of lung tissue sections were observed. Results:In the experimental stage, the arthritis index score of the Tongbi Granule group was finally reduced from 8.25 at the beginning to 5.12.Compared with the model group, the arthritis index scores of the three observation time points were lower (all p<0.05). After 28 days, compared with the model group, the serum TNF-α level (122.59 pg/mL) in the Tongbi Granule group was lower than that in the model group (179.87 pg/mL)(P<0.01). HE and Masson staining observation of lung interstitial tissue morphology of RA-ILD rats showed that the lung interstitial morphology of model rats was significantly changed, and the Tongbi G ranules group was close to the normal group.Conclusion:Tongbi Granule has obvious protective effect on pulmonary interstitial lesions induced by bovine type Ⅱ collagen combined with bleomycin. |
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