To analyze the effects of autohemotherapy on the levels of interleukin-2 (IL-2),IL-6,c-reactive protein (CRP) and procalcitonin (PCT) in rats with chronic obstructive pulmonary disease (COPD) in stable phase.Methods:A total of 60 healthy and clean male Wistar rats were selected and divided into blank control group, blank treatment group and autohemotherapy group by random lottery. Rats in the blank treatment group and the autohemotherapy group were modeled by cigarette smoking combined with intraairway toxin infusion. After modeling, the rats in the blank control group and the blank treatment group were drenched with normal saline, and the rats in the autohemotherapy group were given the autohemotherapy intervention. All rats in the three groups were treated for 30 days, the changes of serum IL-2, IL-6, CRP, PCT, serum matrix metalloproteinase-9 (MMP-9) and metalloproteinase inhibitor 1 (TIMP-1) before and after treatment were compared. Results:After treatment, the levels of IL-2,IL-6,CRP and PCT in the autohemotherapy group were lower than those in the blank treatment group, but was higher than the blank control group(all P<0.05).After treatment, serum levels of MMP-9 and TIMP-1 in rats from the autohemotherapy group were lower than those in the blank treatment group, but was higher than the blank control group (all P<0.05).Conclusion:Autohemotherapy can significantly alleviate the inflammatory response, reduce serum MMP-9 and TIMP-1 of the model rats. It may be one of the effective methods to treat chronic obstructive pulmonary disease in stable phase.