| To study the antitumor effects of Triptolide (TP) used in rats with ovarian cancer and its influence on the immune system.Methods:Female SD rats were selected as experimental animals,and ovarian cancer-bearing rats were obtained after subcutaneous injection of ovarian cancer cells.They were divided into a model group,a low-dose TP group,a middle-dose TP group,and a high-dose TP group injected with physiological saline.After drug intervention,the weight and volume of the tumor,the expression of tumor suppressor genes in the tumor tissue,and the content of immune cytokines in the peripheral blood were measured.Results:Tumor weight and volume,interleukin-4 (IL-4) in peripheral blood,interleukin-17 (IL-17),and transforming growth factor β1 (TGF-β1) in rats in the low-dose,middle-dose,and high-dose TP groups were significantly lower than the model group,and the expression of PTEN,ST7L,Bax,TIMP1,E-cadherin mRNA in tumor tissues and interferon-γ (IFN-γ) and tumor necrosis factor α (TNF-α) in peripheral blood were significantly higher than the model group.The greater the TP dose,the lower the tumor weight and volume,and the content of IL-4,IL-17,and TGF-β1 in the peripheral blood,the higher the mRNA expression of PTEN,ST7L,Bax,TIMP1,E-cadherin in the tumor tissue,and the higher the content of IFN-γ and TNF-α in peripheral blood.Conclusion:TP has a significant tumor suppressing effects on ovarian cancer-bearing rats,which can inhibit tumor growth,increase tumor suppressor gene expression and regulate anti-tumor immune response. |
